三种猕猴桃多酚粗提物对A549和Hela细胞的抑制作用
详细信息Antiproliferative activity of three kiwi polyphenol crude extracts on A549 and Hela cells
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摘要: 使用60%的乙醇提取三种猕猴桃多酚,离心后上清液通过旋转蒸发仪真空浓缩获得猕猴桃多酚粗提物,通过细胞形态分析、MTT实验以及DNALadder实验分析三种猕猴桃粗提物对A549和Hela细胞的抑制作用。结果表明狗枣猕猴桃多酚粗提物具有较好的抗肿瘤增殖活性,因此,软枣猕猴桃多酚可以作为新的癌症抑制剂,具有潜在的应用价值。
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关键词:
- 猕猴桃; /
- 多酚; /
- 抗癌活性; /
- DNALadder;
Abstract: The three kinds of kiwifruit polyphenol were extracted with 60% ethanol. The supernatants after centrifugation were then individually pooled and condensed using a rotary evaporator.The antiproliferative activity of the kiwi crude extracts on A549 and Hela cell was analyzed by cell morphology analysis, MTT assay and DNA Ladder.The results demonstrated that the Actinidia arguta extract had a higher antiproliferative activity than the other two kinds of Actinidia extracts.Therefore, Actinidia arguta extract could be used as a new cancer inhibitor.The results provided new insight into the health function of fruit and demonstrated that Actinidia extracts potentially had health benefits. -
[1] Zong A, Cao H, Wang F.Anticancer polysaccharides from natural resources:A review of recent research[J].Carbohydrate Polymers, 2012, 90 (4) :1395-1410.
[2] Wang X, Wei Y, Yuan S, et al.Potential anticancer activity of litchi fruit pericarp extract against hepatocellular carcinomain vitro and in vivo[J].Cancer Letters, 2006, 239 (1) :144-150.
[3] Zuo L-L, Wang Z-Y, Fan Z-L, et al.Evaluation of Antioxidant and Antiproliferative Properties of Three Actinidia (Actinidia kolomikta, Actinidia arguta, Actinidia chinensis) Extracts in vitro[J].International Journal of Molecular Sciences, 2012, 13 (5) :5506-5518.
[4] Fouche G, Cragg GM, Pillay P, et al.In vitro anticancer screening of South African plants[J].Journal of Ethnopharmacology, 2008, 119 (3) :455-461.
[5] Zhou H-C, Lin Y-M, Wei S-D, et al.Structural diversity and antioxidant activity of condensed tannins fractionated from mangosteen pericarp[J].Food Chemistry, In Press, Corrected Proof.
[6] Prasad KN, Hao J, Shi J, et al.Antioxidant and anticancer activities of high pressure-assisted extract of longan (Dimocarpus longan Lour.) fruit pericarp[J].Innovative Food Science&Emerging Technologies, 2009, 10 (4) :413-419.
[7] Yang J, Liu RH.Induction of phase II enzyme, quinone reductase, in murine hepatoma cells in vitro by grape extracts and selected phytochemicals[J].Food Chemistry, 2009, 114 (3) :898-904.
[8] Feng R, Ni HM, Wang SY, et al.Cyanidin-3-rutinoside, a natural polyphenol antioxidant, selectively kills leukemic cells by induction of oxidative stress[J].Journal of Biological Chemistry, 2007, 282 (18) :13468-13476.
[9] Fiorentino A, D'Abrosca B, Pacifico S, et al.Identification and Assessment of Antioxidant Capacity of Phytochemicals from Kiwi Fruits[J].Journal of Agricultural and Food Chemistry, 2009, 57 (10) :4148-4155.
[10] Piao MJ, Yoo ES, Koh YS, et al.Antioxidant Effects of the Ethanol Extract from Flower of Camellia japonica via Scavenging of Reactive Oxygen Species and Induction of Antioxidant Enzymes[J].International Journal of Molecular Sciences, 2011, 12 (4) :2618-2630.
[11] 葛锡娟, 许岩, 杨丽丽, 等大豆抗癌肽的初步提取及抗癌活性[J].食品科学, 2011, (16) :27-30. [12] Gooch JL, Yee D.Strain-specific differences in formation of apoptotic DNA ladders in MCF-7breast cancer cells[J].Cancer Letters, 1999, 144 (1) :31-37.
[13] Daniel PT, Sturm I, Ritschel S, et al.Detection of Genomic DNA Fragmentation during Apoptosis (DNA Ladder) and the Simultaneous Isolation of RNA from Low Cell Numbers[J].Analytical Biochemistry, 1999, 266 (1) :110-115.
[14] Huang GJ, Deng JS, Huang SS, et al.Hispolon Induces Apoptosis and Cell Cycle Arrest of Human Hepatocellular Carcinoma Hep3B Cells by Modulating ERK Phosphorylation[J].Journal of Agricultural and Food Chemistry, 2011, 59 (13) :7104-7113.
[15] Zou YP, Chang SKC.Effect of Black Soybean Extract on the Suppression of the Proliferation of Human AGS Gastric Cancer Cells via the Induction of Apoptosis[J].Journal of Agricultural and Food Chemistry, 2011, 59 (9) :4597-4605.
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