响应面法优化罗非鱼鱼皮胶原多肽螯合镁的工艺条件的研究
详细信息Optimization of process conditions for tilapia skin collagen peptides chelating with magnesium by response surface methodology
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摘要: 以罗非鱼鱼皮为原料,研究鱼皮胶原多肽和镁离子的螯合工艺条件。探讨了鱼皮胶原多肽与氯化镁的质量比、反应体系的pH、螯合温度、螯合时间等因素对鱼皮胶原蛋白螯合反应的影响;在单因素实验的基础上,通过响应面法对鱼皮胶原多肽与镁的螯合工艺条件进行优化,确定最佳的螯合工艺条件为:质量比1∶5、pH7.0、螯合温度70℃、螯合时间30min。在此条件下,螯合率达84.58%。通过对比螯合反应前后傅里叶变换红外光谱图的变化,证明了鱼皮胶原多肽与镁离子形成了新的螯合物。Abstract: With Tilapia skin as material, chelating conditions for skin collagen peptides and magnesium ( II) iron were investigated.The effects of collagen peptides - magnesium chloride ratio, pH, tempretureand reaction time on chelating efficiency were discussed. According to single test and response surface experiment, the optimum preparation conditions of collagen peptides - magnesium chloride were obtained as follows: collagen peptides / magnesium mass ratio of 1∶5, pH7.0, temperature 70℃, reaction time 30min.Under these conditions, the chelation rate was up to 84.58%.The results from fourier transform infrared spectroscopy scan also proved that the collagen peptides of Tilapia fish skin and magnesium ions were chaleted and turned into a new kind of chelate compoud.
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[1] 秦刚, 王庭.罗非鱼下脚料鱼糜系列功能性产品的研发[J].肉类研究, 2010, 137 (7) :78-81. [2] 霍健聪, 邓尚贵, 谢超.带鱼下脚料蛋白多肽铁螯合物的制备及抗氧化活性研究[J].食品工业科技, 2009, 30 (4) :267-270. [3] 张俊敏, 乐辉, 陈忻, 等.响应面法优化微波固相合成鳕鱼皮胶原蛋白锌肽螯合物的工艺[J].广东农业科学, 2011, (22) :87-90. [4] 陆剑锋, 孟昌伟, 李进, 等.斑点叉尾鮰鱼骨胶原多肽螯合钙的制备及其特征[J].水产学报, 2012, 36 (2) :314-319. [5] 许庆陵, 曾庆祝, 闫磊, 等.罗非鱼肽-锌配合物的制备及其生物活性[J].食品科学, 2010, 31 (10) :75-80. [6] 黄晓钰, 刘邻渭.食品化学综合实验[M].北京:中国农业大学出版社, 2002:131-132. [7] Gums JG.Magnesium in cardiovascular and other disorders[J].Am J Health Syst Pharm, 2004, 61 (15) :1569-1576.
[8] 程春萍, 张丽娜.连续滴定法测定蛋壳中钙、镁离子的含量[J].内蒙古石油化工, 2010 (8) :35-36. [9] 杨姗姗.罗非鱼皮多肽锌螯合盐的制备及性质研究[M].南昌:南昌大学, 2008. [10] Wedekind K J A E Hortin, DH Baker.Methodology forassessing Zinc bioavailability efficacy estimates for Zinc-2methionihe, Zinc Sulfate, and Zinc Oxide[J].J A nim Sci, 1992, 70:178-187.
[11] 巴索洛, 皮尔逊.无机反应原理-溶液重金属络合物的研究[M].1987, 5. [12] 李彤刚, 洪鹏志, 杨萍, 等.二次回归正交旋转组合优化罗非鱼下脚料超滤酶解液螯合锌的工艺条件[J].食品工业科技, 2013, 34 (2) :234-237. [13] 钟明杰.带鱼下脚料蛋白水解螯合物制备及生物特性研究[D].青岛:中国海洋大学, 2009. [14] 付文雯, 马美湖, 蔡朝霞.牛骨蛋白酶解制取肽钙的研究进展[J].食品与发酵科技, 2009, 45 (1) :1-5. [15] 张晓霞, 刘剩取, 李国英.碱法水解黑鱼鱼鳞及制备多肽螯合钙工艺的研究[J].食品科技, 2010, 35 (12) :130-134. [16] 孙胜玲, 王爱勤, 高忆慈, 等.D-氨基葡萄糖与锌盐配位的红外光谱研究[J].光谱学与光谱分析, 2005, 25 (3) :374-376. [17] E P Paschalis, O Jacenko, B Olsen, et al.Fourier transform infrared microspectroscopic analysis identifies alterations in mineral properties in bones from mice transgenic for type X collagen[J].Bone, 1996, 2 (19) :151-156.
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