响应面法优化罗非鱼鱼皮胶原多肽螯合镁的工艺条件的研究

Optimization of process conditions for tilapia skin collagen peptides chelating with magnesium by response surface methodology

摘要: 以罗非鱼鱼皮为原料,研究鱼皮胶原多肽和镁离子的螯合工艺条件。探讨了鱼皮胶原多肽与氯化镁的质量比、反应体系的pH、螯合温度、螯合时间等因素对鱼皮胶原蛋白螯合反应的影响;在单因素实验的基础上,通过响应面法对鱼皮胶原多肽与镁的螯合工艺条件进行优化,确定最佳的螯合工艺条件为:质量比1∶5、pH7.0、螯合温度70℃、螯合时间30min。在此条件下,螯合率达84.58%。通过对比螯合反应前后傅里叶变换红外光谱图的变化,证明了鱼皮胶原多肽与镁离子形成了新的螯合物。

Abstract: With Tilapia skin as material, chelating conditions for skin collagen peptides and magnesium ( II) iron were investigated.The effects of collagen peptides - magnesium chloride ratio, pH, tempretureand reaction time on chelating efficiency were discussed. According to single test and response surface experiment, the optimum preparation conditions of collagen peptides - magnesium chloride were obtained as follows: collagen peptides / magnesium mass ratio of 1∶5, pH7.0, temperature 70℃, reaction time 30min.Under these conditions, the chelation rate was up to 84.58%.The results from fourier transform infrared spectroscopy scan also proved that the collagen peptides of Tilapia fish skin and magnesium ions were chaleted and turned into a new kind of chelate compoud.