海带多糖纯化及清除自由基活性研究

Purification and radical scavenging effect of Laminaria japonica polysaccharide

摘要: 海带多糖经S-300凝胶层析分离后得到纯化组分WPS-1-1。采用高效液相色谱、红外光谱对WPS-1-1结构进行解析,结果表明,WPS-1-1分子量为112ku,主要由岩藻糖、木糖、半乳糖和甘露糖构成,其摩尔比为6.9∶3.3∶1.5∶1。WPS-1-1含有β-吡喃糖苷键和硫酸基。体外自由基清除实验结果表明,WPS-1-1具有超氧阴离子自由基和羟自由基清除活性,其IC50分别为0.35、1.0mg/mL,可作为天然的功能性自由基高效清除剂。

Abstract: A purified polysaccharide of WPS-1-1 was obtained from crude extracts of Laminaria japonica by S-300 gel chromatography.The average molecular weight of WPS-1-1 was 112ku. Monosaccharides analysis revealed that WPS-1-1 was composed of fucose, xylose, galactose and mannose with a molar ratio of 6.9∶3.3∶1.5∶1. The results of infrared spectrum indicated that WPS-1-1 contained β-pyranose and sulfate group. In vitro, radical scavenging tests revealed that WPS-1-1 showed significant function of scavenging superoxide and hydroxyl radicals with IC 50 value of 0.35 and 1.0mg /mL.It could be a potential functional free-radical scavenger.