水解法制备石榴皮鞣花酸工艺研究

doi:10.13386/j.issn1002-0306.2013.12.041

水解法制备石榴皮鞣花酸工艺研究

详细信息

中图分类号: TS209
计量
- 文章访问数: 141
- HTML全文浏览量: 20
- PDF下载量: 488
出版历程
- 收稿日期: 2013-01-14

Study on preparation of ellagic acid by hydrolysis from pomegranate peel

摘要

摘要: 建立了以60%乙醇超声提取石榴皮粉末,粗提物经石油醚萃取除去脂溶性物质,乙酸乙酯萃取,萃取物用酸水解法水解制备鞣花酸的方法。采用超高效液相色谱-质谱法（UPLC-MS）确定了乙酸乙酯萃取部位的主要成分为粟木鞣花素、安石榴苷、石榴亭A、鞣料云石素、石榴亭B、鞣花酸,并鉴定酸水解产物为鞣花酸。采用毛细管电泳法（CE）监测了乙酸乙酯萃取物的酸水解历程,结果表明,随着水解时间的增加,乙酸乙酯萃取物的电泳峰不断减少,而水不溶物鞣花酸的质量不断增加。正交实验考察了水解时间、水解温度、酸浓度对鞣花酸得率的影响,最优水解条件为:水解时间6h、温度100℃、硫酸浓度1.0mol/L,鞣花酸得率达32.7%。
- 石榴皮; /
- 鞣花酸; /
- 酸水解; /
- 毛细管电泳; /
- 超高效液相色谱-质谱;
Abstract: Preparation of ellagic acid from pomegranate peel by hydrolysis was developed. Pomegranate peel powder was extracted with 60% ethanol by means of ultrasonic -assisted extraction to obtain crude extract. After removal of lipophilic ingredients by extracting with petroleum ether, crude extract was extracted with ethyl acetate, and the ethyl acetate fraction was hydrolyzed to produce ellagic acid. With the ultra performance liquid chromatography-mass spectrometry, the main ingredients in the ethyl acetate fraction were identified as castalagin, punicalagin, granatin A, corilagin, granatin B and ellagic acid, and the hydrolysis product was identified as ellagic acid. The hydrolysis process of the ethyl acetate fraction was monitored by capillary electrophoresis. It had been found that with the increase of hydrolysis time, the number of peaks in electropherogams was reduced, while the weight of ellagic acid increasing. With the orthogonal experiment, the influences of hydrolysis time, hydrolysis temperature, as well as acid concentration on the ellagic acid yield was investigated. It turned out that optimum conditions of hydrolysis were as follows:hydrolysis time of 6h, hydrolysis temperature at 100℃, and sulfuric acid of 1.0mol/L, with the ellagic acid yield of 32.7%.
- pomegranate peel;ellagic acid;acid hydrolysis;CE;UPLC-MS; /
- undefined /
- undefined /
- undefined /
- undefined

HTML全文

参考文献(22)

[1]	李海霞, 王钊, 刘延泽.石榴科植物化学成分及药理活性研究进展[J].中草药, 2002, 33 (8) :765-769.
[2]	张建成, 屈红征, 张晓伟.中国石榴的研究进展[J].河北林果研究, 2005, 20 (3) :265-272.
[3]	王晓瑜, 高晓黎, 买尔旦·马合木提.石榴的药理学研究进展[J].中国医药导报, 2008, 5 (7) :13-15.
[4]	Negi P S, Jayaprakasha G K, Jena B S.Antioxidant and antimutagenic activities of pomegranate peel extracts[J].Food Chemistry, 2003, 80:393-397.
[5]	Tariq I, Piero S, Saeed A.Pomegranate peel and fruit extracts:A review of potential anti-inflammatory and anti-infective effects[J].Journal of Ethnopharmacology, 2012, 143:397-405.
[6]	Li Y F, Guo C J, Yang J J, et al.Evaluation of antioxidan properties of pomegranate peel extract in comparison with pomegranate pulp extract[J].Food Chemistry, 2006, 96:254-260
[7]	AlZoreky N S.Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels[J].International Journal of Food Microbiology, 2009, 134:244-248.
[8]	Ephraim P L, Robert A N.Punica granatum (pomegranate) and its potential for prevention and treatment of inflammationand cancer[J].Journal of Ethnopharmacology, 2007, 109:177-206.
[9]	Ali T, Yahya S, Mahdiyeh K, et al.High potential of agro-industrial by-products of pomegranate (Punica granatum L.) as the powerful antifungal and antioxidant substances[J].Industrial Crops and Products, 2011, 34:1523-1527.
[10]	Faten Z, Pedro M, Cristina G V, et al.Antioxidant activity and physico-chemical properties of Tunisian grown pomegranate (Punica granatum L.) cultivars[J].Industrial Crops and Products, 2012, 40:81-89.
[11]	李婕姝, 贾冬英, 姚开, 等.石榴的生物活性成分及其药理作用研究进展[J].中国现代中药, 2009, 11 (9) :7-10.
[12]	Navindra P S, Rupo L, David H.Bioavailability of ellagic acid in human plasma after consumption of ellagitannins from pomegranate (Punica granatum L.) juice[J].Clinica Chimica Acta, 2004, 348:63-68.
[13]	Maria A R, Marina S H, Ana C, et al.Dietary supplementation of an ellagic acid-enriched pomegranate extract attenuates chronic colonic inflammation in rats[J].Pharmacological Research, 2012, 66:235-242.
[14]	Dhiraj A V, Kalidas S.Ellagic acid production and phenolic antioxidant activity in cranberry pomace (Vaccinium macrocarpon) mediated by Lentinus edodes using a solid-state system[J].Process Biochemistry, 2003, 39:367-379.
[15]	Lei F, Xing D M, Xiang L, et al.Pharmacokinetic study of ellagic acid in rat after oral administration of pomegranate leaf extract[J].Journal of Chromatography B, 2003, 796:189-194.
[16]	Soong Y Y, Philip J B.Quantification of gallic acid and ellagic acid from longan (Dimocarpus longan Lour.) seed and mango (Mangifera indica L) .kernel and their effects on antioxidant activity[J].Food Chemistry, 2006, 97:524-530.
[17]	李庆, 姚开, 谭敏.新型天然抗氧化剂—鞣花酸[J].四川食品与发酵, 2001, 37 (4) :10-14.
[18]	李素琴, 袁其朋, 徐健梅.鞣花酸的生理功能及工艺开发研究现状[J].天然产物研究与开发, 2001, 13 (5) :71-74.
[19]	王妙飞, 程庚金生, 张道英, 等.水解法制取五倍子鞣花酸的研究[J].食品工业科技, 2010, 31 (2) :233-234.
[20]	纪白慧, 倪鑫炯, 曹玉华.石榴皮抗氧化活性成分的提取及其组分的研究[J].天然产物研究与开发, 2012 (24) :17-22.
[21]	郭珊珊.石榴中类单宁的分离纯化、结构及活性研究[D].武汉:华中农业大学, 2007.
[22]	Maryam Z, Farrukh A, Iqbal A.Broad spectrum antimutagenic activity of antioxidant active fraction of Punica granatum L.peel extracts[J].Mutation Research, 2010, 703:99-107.

施引文献(9)

期刊类型引用(3)

1.	卢秋玲，李琅，杨敏，魏彦明. 超声处理对文冠果种粕蛋白结构及性质的影响. 食品与发酵工业. 2024(20): 79-87 . 百度学术
2.	何凤平，张燕，王代谷，康专苗，李向勇，朱文华. 澳洲坚果牛轧糖的制作工艺. 食品工业. 2022(12): 91-95 . 百度学术
3.	薄璇，杨婧，侯艳霞. 红枣核桃牛轧软糖制作工艺研究. 中国果菜. 2020(05): 45-49 . 百度学术