QuEChERS-HPLC快速测定食品中七种食用合成色素
详细信息QuEChERS-high performance liquid chromatography for the determination of synthetic colorants in food
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摘要: 建立了一种同时测定豆制品和肉制品中7种食用合成色素（柠檬黄、日落黄、胭脂红、苋菜红、亮蓝、偶氮玉红、诱惑红）的QuEChERS-HPLC方法。样品采用乙醇-氨水-水（7∶2∶1）超声提取,混合使用C18和乙二胺-N-丙基硅烷（PSA）两种基质分散净化剂净化,浓缩后采用SunFireTMC18色谱柱（250mm×4.6mm,5μm）分离。以甲醇-20mmol/L乙酸铵水溶液为流动相,梯度洗脱,流速为1mL/min,测定波长410nm和520nm。实验通过空白基质溶液稀释标准品建立校正的标准曲线,以消除基质效应。结果表明,7种食用合成色素在0.5～10μg/mL线性范围内具有良好的线性关系（相关系数0.9971～0.9999）,样品加标回收率为83.01%～108.84%,相对标准偏差为4.53%～15.23%,方法的检出限在0.1～0.3mg/L之间,定量限在0.3～0.4mg/kg之间。方法操作简便、快速、准确,可满足豆制品和肉制品中食用合成色素的分析要求。Abstract: A high performance liquid chromatographic method was developed for the simultaneous determination of 7 synthetic colours such as tartrazine, sunset yellow, ponceau 4R, amaranth, brilliant blue, carmoisine, allura red in bean and meat products. The analytes were extracted from the samples by ethanol-ammonia -water (volume ratio 7∶2∶1) , and purified by a modified QuEChERS (quick, easy, cheap, effective, rigged, and safe) method, and then separated by a SunFire TM C 18 (250mm×4.6mm, 5μm) after concentrating. Methanol-20mmol/L ammonium acetate was used as mobile phase with a gradient elution at the rate of 1mL/min and the detection wavelength was 410nm and 520nm. The interference of matrix was reduced by the matrix-matched calibration standard curves. The linear range was 0.5～10μg/mL with correlation coefficients from 0.9971 to 0.9999. When 0.5～10μg/mL standard solutions were added to blank samples, the average recoveries were 83.01%～108.84% and the relative standard deviations ranged from 4.53% ～15.23% . The limits of detection were ranged from 0.1mg/L to 0.3mg/L and the limit of quantification from 0.3mg/kg to 0.4mg/kg. This method is simple, accurate and sensitive to screen synthetic colorants residue in bean and meat products.
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Keywords:
- QuEChERS;HPLC;synthetic colorants; /
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