响应面法优化核桃壳总黄酮提取工艺的研究
详细信息Optimization of the extraction of total flavonoids from Walnut shell using response surface methodology
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摘要: 利用响应面法对核桃壳总黄酮的提取工艺进行优化。在单因素实验基础上,以提取温度、乙醇浓度和液固比为自变量,总黄酮提取量为响应值,以响应面分析法,研究各自变量交互作用及其对总黄酮提取的影响,模拟得到二次多项式回归方程,进一步得到核桃壳总黄酮的最佳提取工艺:乙醇浓度59%,提取温度为78℃,液固比为33:1。验证实验得到核桃壳总黄酮含量为3.61mg·g-1,与预测值相吻合。Abstract: In this paper, the optimum conditions for the extraction of total flavonoids from Walnut shell were determined using response surface methodology. On the basis of single-factor experiments, selecting extraction temperature, ethanol concentration and liquid-to-solid ratio as independent variables, response surface methodology was applied to investigate the effect of three variables on extraction yield of total flavonoids. A regression model describing extraction content of total flavonoids as a function of these three variables was established. The optimal conditions for achieving the high extraction rate of total flavonoids from Walnut shell were ethanol concentration of 59%, extraction temperature of 78℃ and liquid-to-solid ratio of 33:1. The result of verification experiment was coincided with predictive value, the content of total flavonoids from Walnut shell was 3. 61mg·g-1.
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