鲢肉蛋白酶水解工艺优化的研究
详细信息Optimizing conditions for enzymatic hydrolysis of silver carp protein
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摘要: 以鲢肉为研究对象,研究采用不同蛋白酶水解白鲢鱼肉对酶解产物的氮收率、水解度、平均肽链长度的影响,并对酶解条件进行优化。结果表明,碱性蛋白酶可以获得较高的氮收率、水解度和平均肽链长度,是最合适的酶;碱性蛋白酶的最佳酶解条件为温度55℃、酶浓度2000U/g、pH7.5、料液比1∶4。酶解3h时,鲢肉蛋白酶解液的平均肽链长度为3.87,水解度为24.12%,氮收率为91.85%。Abstract: The enzymatic hydrolysis action of protein in silver carp by different proteases was investigated. The nitrogen yield, degree of hydrolysis and average length of peptide chain were studied on enzymatic hydrolysate. Results showed that Alcalase was the best that had higher nitrogen yield, degree of hydrolysis and average length of peptide chain. The optimum enzymatic conditions for Alcalase hydrolysis were that hydrolyzing at 55℃, adding 2000U/g, pH7.5 and feed ratio of 1∶4. The average lengths of peptide chain, degree of hydrolysis, nitrogen yield were 3.87, 24.12% and 91.85% respectively after hydrolysis of 3h.
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Keywords:
- silver carp;enzyme hydrolysis;optimizing; /
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