金属硫蛋白对鲤鱼不同组织重金属蓄积影响的研究
详细信息Study on metallothionein impact for heavy metal accumulation of different organizations in carp
-
摘要: 金属硫蛋白(Metallothionein,简MT)能有效地表征水体中重金属对生物的影响,故以MT作为生物标志物,采用ELISA法测定鲤鱼组织中MT的含量,以鲤鱼各组织中MT的含量动态变化来评价在其受重金属胁迫下不同组织对重金属的蓄积规律。探讨鱼体内MT与水环境和体内组织中重金属之间的相关性。对深入研究鱼体自身对重金属的蓄积和净化机制、对不同重金属耐受力、确定污染源及污染程度等具有重要意义。Abstract: Metallothionein ( MT) could effectively represent the influences heavy metal in water has on creatures, so used MT as the biomarker and ELISA to measure the MT content in the tissue of carp.Then evaluated accumulation law in different tissue under the stress of heavy metal by the dynamic changes of MT content in carp tissues, and discussed the correlation between the MT in carp and heavy metal in water and carp tissues. The study was of significance for further research about the carp ’s accumulation and removal mechanism of heavy metal, its tolerance of different kinds of heavy metal, and the ascertainment of pollution source and level.
-
[1] Linde A R, S Sanchez-Galan, Klein D, et al.Metallothionein and heavy meatals in brown trout (Salmo trutta) and European eel (Anguilla anguilla) :A comparative study[J].Ecotoxicology and environmental safety, 1999, 44:168-173.
[2] Syllviane L G, Nicole M G.Invitro dose-response study of theeffect of cadmium on eel (Anguilla anguilla) gill Na+/K+-ATPase Activities[J].Ecotoxicology and environmental safety, 1994, 28:43-52.
[3] Allen P.Chronic accumulation of cadmium in the edible tissues of Oreochromis aureus (Steindachner) :Modification by Mercury and Lead.Arch Environ[J].Contam toxicol, 1995, 29:8-14.
[4] 邹淑美, 秦学祥.海洋生物对无机污染物的吸收、富集和转移[J].海洋环境科学, 1990, 3:59-61. [5] Maiti, Banerjee S.Accumulation of heavy metals in different tissues of the fish Oreochrom is nilotica exposed to waste water[J].Environ Ecol, 1999, 17 (4) :895-898.
[6] Kagi J H R, Schaffer A.Biochemistry of metallothionein[J].Biochemistry, 1988, 27:8509-8511.
[7] 王海黎, 陶澍.生物标志物在水环境研究中的应用[J].中国环境科学, 1999, 19 (5) :421-426. [8] Raspor B, Pavicic J, Branica M, et al.Water aguatic determination of the act lerhal toxicity of substandes to a freshwater fish[J].Anal Chem, 1987, 326 (7) :719.
[9] Robert J.Cousins, measurement of humen metallothionein by enzyme linked immunosorbent assay[J].Methods in Enzymology, 2005 (Part B) :131-140.
[10] Ovemell J, Abdullah M I.Metallothionein and metal levels in flounder platichthys flesus from four field sites and in flounder dosed with water brone copper[J].Mar Ecol Prog Ser, 1988, 46:71-74.
[11] Rainbow P S.海洋生物对重金属的积累及意义[J].海洋环境科学, 1992, 11 (1) :44-52. [12] 吴玉霖, 崔可铎, 赵鸣儒, 等.渤海无脊椎动物体内痕量金属含量的研究[J].海洋与湖沼, 1986, 17 (6) :539-547. [13] Dethloff G M, Schlenk D, Hamm J T, et al.Alterations Alterations in physiological parameters of rainbow trout (Oncorhynchus mykiss) with exposure to copper and copper/zinc mixtures[J].Ecotoxicol Environ Saf, 1999 (42) :253-264.
[14] Marr J C A, Bergman H L, Lipton J, et al.Differences in relative sensitivity of naive and metals-acclimated brown and rainbow trout exposed to metals representative of the C lark Fork River, Montana[J].Can J Fish Aquat Sci, 1995, 52:2016-2030.
[15] Dursu S D, Khan S, Bailey H C.The effects of copper on blood and biochemical parameters of rainbow trout (Oncorhynchus mykiss) [J].Arch Environ Contam Toxicol, 1999, 36:415-423.
[16] 韩照祥, 胡喜兰, 王庆祝.重金属在鱼体内的蓄积及其防御机制研究[J].水利渔业, 2006, 26 (5) :147-212. -
期刊类型引用(11)
1. 王敬涵，王舒彤，戴瑞，杜密英，胡燕，武家宇，赵冬. 芋头粉对低盐巴沙鱼鱼糜凝胶特性的影响. 中国调味品. 2024(08): 106-112 . 
百度学术
2. 张学文，曾艳，董婷，孟洁，孙媛霞. 菌丝蛋白对仿肉品质特性的影响. 食品研究与开发. 2023(03): 26-31 . 百度学术
3. 韦芳，窦容容，孙纪录，桑亚新，亢春雨，赵春青. 鱼糜制品凝胶特性影响因素的研究进展. 食品研究与开发. 2023(19): 208-215 . 百度学术
4. 洪金玲，何慧琪，翁武银，姜泽东，倪辉，郑明静. 不同粒径的柚子皮全粉对鱼丸品质特性和蛋白二级结构的影响. 中国食品学报. 2022(07): 123-132 . 百度学术
5. 李卫涛，康东亮，王焰. 沙拉鱼糜食品的加工工艺及仓储稳定性研究. 中国调味品. 2022(12): 120-123+147 . 百度学术
6. 薛山，谢建山. 基于质构优化蜜柚白瓤水不溶性膳食纤维兔肉丸制作配方. 食品工业科技. 2021(05): 138-145+151 . 本站查看
7. 何丹，申铉日. 营养强化型鱼糜制品研究进展. 肉类研究. 2021(09): 58-63 . 百度学术
8. 陈丽，饶杰，赵永强，杨贤庆，魏涯，李春生，王悦齐. 坛紫菜膳食纤维的响应面优化超声复合酶法提取及其对鱼糜凝胶强度的影响. 大连海洋大学学报. 2021(05): 796-805 . 百度学术
9. 薛山. 基于质构品质降维分析法优化番茄皮渣鱼丸加工工艺. 肉类研究. 2020(01): 51-58 . 百度学术
10. 仪淑敏，吕柯明，张诗雯，励建荣，李学鹏，徐永霞，牟伟丽，沈琳，谢晶，郭晓华，于建洋. 芹菜叶改善鱼糕的凝胶特性. 现代食品科技. 2020(02): 152-158+52 . 百度学术
11. 李可，李燕，刘俊雅，赵颖颖，刘骁，赵卫东，白艳红. 竹笋膳食纤维结合预乳化植物油对低脂猪肉糜乳化稳定性和流变学特性的影响. 食品与发酵工业. 2020(20): 9-14 . 百度学术
其他类型引用(7)
计量
- 文章访问数: 114
- HTML全文浏览量: 13
- PDF下载量: 265
- 被引次数: 18
下载:
