真菌毒素免疫分析中新型检测元件的研究进展

doi:10.13386/j.issn1002-0306.2013.03.060

真菌毒素免疫分析中新型检测元件的研究进展

基金项目:

国家自然科学基金资助(31171696、31201360)；食品科学与技术国家重点实验室自由探索资助项目(SKLF-TS-201110)；

详细信息

中图分类号: TS207.4
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- 收稿日期: 2012-07-31

Research progress in novel materials for mycotoxin immunoassay

摘要

摘要: 真菌毒素免疫分析是基于抗体与真菌毒素间的相互作用。虽然具有高亲和力及特异性的抗体制备技术已非常成熟,但代替传统抗体或真菌毒素的新型检测元件越来越受到人们的关注。这些元件分为以重组抗体片段为代表的重组类检测元件,如单链抗体,以化学法人工合成的合成类检测元件,如分子印迹聚合物,及其他用于取代真菌毒素标准品的检测元件,如抗独特型抗体。本文重点综述了新型真菌毒素检测元件的研究进展,及其在真菌毒素免疫分析中的应用前景。
- 真菌毒素; /
- 免疫分析; /
- 重组抗体; /
- 分子印迹聚合物; /
- 抗独特型抗体;
Abstract: Mycotoxin immunoassays depend on interactions between antibodies and mycotoxins. Although intact antibodies with high affinity and specificity were well developed, novel materials instead of conventional antibodies or mycotoxins for immunoassays attracted more and more attention. The alternatives can be classified into those that are represented by recombinant antibodies and their fragments such as single chain variable fragments, and those that are obtained by chemical synthesis such as molecularly imprinted polymers, and those that are used to replace mycotoxin standards such as anti- idiotypic antibodies.Recent developments of novel materials and their potential for application in mycotoxin immunoassays were reviewed.
- mycotoxin;immunoassay;recombinant antibody;molecularly imprinted polymer;anti-idiotypic antibody; /
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参考文献(47)

[1]	BALAZS E, SCHEPERS J S.The mycotoxin threat to food safety[J].International Journal of Food Microbiolology, 2007, 119 (1-2) :1-2.
[2]	PEREZ-TORRRADO E, BLESA J, MOLTO J C, et al.Pressurized liquid extraction followed by liquid chromatography-mass spectrometry for determination of zearalenone in cereal flours[J].Food Control, 2010, 21 (4) :399-402.
[3]	SOARES C, RODRIGUES P, FREITAS-SILVA O, et al.HPLC method for simultaneous detection of aflatoxins and cyclopiazonic acid[J].World Mycotoxin Journal, 2010, 3 (3) :225-231.
[4]	TURNER N W, SUBRAHMANYAM S, PILETSKY S A.Analytical methods for determination of mycotoxins:a review[J].Analytica Chimica Acta, 2009, 632 (2) :168-180.
[5]	LUO J, OBMOLOVA G, HUANG A.et al.Coevolution of antibody stability and vκcdr-l3canonical structure[J].Journal of Molecular Biology, 2010, 402 (4) :708-719.
[6]	VEHNIAINEN M, LEIVO J, LAMMINMAKI U.Directed antibody-engineering techniques and their applications in food immunoassays[J].Trends in Analytical Chemistry, 2011, 30 (2) :219-226.
[7]	赵泽文, 史常旭.单链抗体及其在肿瘤诊疗中的应用[J].华南国防医学杂志, 2009, 23 (4) :73-76.
[8]	LAUER B, OTTLEBEN I, JACOBSEN H J, et al.Production ofa single-chain variable fragment antibody against fumonisin b1[J].J Agric Food Chem, 2005, 53:899-904.
[9]	CHOI G.H, LEE D H, MIN W K, et al.Cloning, expression, and characterization of single-chain variable fragment antibody against mycotoxin deoxynivalenol in recombinant Escherichia coli[J].Protein Expression and Purification, 2004, 35:84-92.
[10]	MIN W K, CHO Y J, PARK J B, et al.Production and characterization of monoclonal antibody and its recombinant single chain variable fragment specific for a food-born mycotoxin, fumonisin B1[J].Bioprocess and Biosystems Engineering, 2010, 33 (1) :109-115.
[11]	SCHIER R, MCCALL A, ADAMS G P, et al.Isolation of picomolar affinity anti-c-erbb-2single-chain fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site[J].Journal of Molecular Biology, 1996, 263 (4) :551-567.
[12]	ZAHND C, SPINELLI S, LUGINBHL B, et al.Directed in vitro evolution and crystallographic analysis of a peptide-binding single chain antibody fragment (scfv) with low picomolar affinity[J].Journal of Biological Chemistry, 2004, 279:18870-18877.
[13]	CHANG H J, CHOI S W, CHUN H S.Expression of functional single-chain variable domain fragment antibody (scfv) against mycotoxin zearalenone in Pichia pastoris[J].Biotechnology letters, 2008, 30 (10) :1801-1806.
[14]	LIU D, WANG C, LI C, et al.Production and characterization of a humanized single-chain antibody against human integrinαvβ3protein[J].Journal of Biochemistry, 2011, 286 (27) :24500-24507.
[15]	SONODA H, KUMADA Y, KATSUDA T, et al.Functional expression of single-chain fv antibody in the cytoplasm of Escherichia coli bythioredoxin fusion and co-expression of molecular chaperones[J].Protein Expression and Purification, 2010, 70 (2) :248-253.
[16]	KUDOU M, EJIMA D, SATO H, et al.Refolding single-chainantibody (scfv) using lauroyl-l-glutamate as a solubilization detergent and arginine as a refolding additive[J].Protein Expression and Purification, 2011, 77 (1) :68-74.
[17]	PAOLETTI F, MALERBA F, KONAREV P, et al.Direct intracellular selection and biochemical characterization of a recombinant anti-proNGF single chain antibody fragment[J].Archives of Biochemistry and Biophysics, 2012, 552 (1) :26-36.
[18]	杨珂, 王冬.纳米抗体及其应用[J].细胞与分子免疫学杂志, 2008, 24 (4) :425-427.
[19]	DOYLEA P J, ARBABI-GHAHROUDI M, GAUDETTE N, et al.Cloning, expression, and characterization of a single-domain antibody fragment with affinity for15-acetyl-deoxynivalenol[J].Molecular Immunology, 2008, 45:3703-3713.
[20]	VAN HOUWELINGEN A, SAEGER S D, RUSANOVA T, et al.Generation of recombinant alpaca vhh antibody fragments for the detection of the mycotoxin ochratoxin A[J].World Mycotoxin Journal, 2008, 1 (4) :407-417.
[21]	涂追.基于单域重链抗体的天然噬菌体文库的构建及抗DON适配体的淘选[D].南昌:南昌大学, 2010.
[22]	WESOLOWSKI J, ALZOGARAY V, REYELT J, et al.Single domain antibodies:promising experimental and therapeutic tools in infection and immunity[J].Med Microbiol Immunol, 2009, 198:157-174.
[23]	DE GAST G C, VAN DE WINKEL J G J, BAST B E.Clinical perspectives of bispecific antibodies in cancer[J].Cancer Immunol Immunother, 1997, 45:121-123.
[24]	WANG Shihua, ZHENG Cengjie, LIU Ying, et al.Construction of multiform scfv antibodies using linker peptide[J].Journal of Genetics and Genomics, 2008, 35 (5) :313-316.
[25]	HAYHURST A, HAPPE S, MABRY, R, et al.Isolation and expression of recombinant antibody fragments to the biological warfare pathogen Brucella melitensis[J].Journal of Immunological Methods, 2003, 276:185-196.
[26]	ZHANG J, YUN J, SHANG Z, et al.Design and optimization of a linker for fusion protein construction[J].Progress in Nature Science, 2009, 19 (10) :1197-1200.
[27]	WULLF G., SARHAN A, ZABROCKI K.Enzyme-analogue built polymers and their uses for the resolution of racemates[J].Tetrahedron Letters, 1973, 44:4329-4332.
[28]	PASCALE M, DE GIROLAMO A, VISCONTI A, et al.Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis[J].Analytica Chimica Acta, 2008, 609:131-138.
[29]	TURNER N W, PILETSKA E V, KARIM K, et al.Effect of the solvent on recognition properties of molecularly imprinted polymer Specific for ochratoxin a[J].Biosensors and Bioelectronics2004, 20:1060-1067.
[30]	GUO B, WANG S, REN B, et al.Citrinin selective molecularly imprinted polymers for spe[J].Journal of Seperation Science, 2010, 33 (8) :1156-1160.
[31]	ALI W H, DERRIEN D, ALIX F, et al.Solid-phase extraction using molecularly imprinted polymers for selective extraction of a mycotoxin in cereals[J].Journal of Chromatography A, 2010, 1217:6668-6673.
[32]	PASCALE M, DE GIROLAMO A, VISCONTI A, et al.Use of itaconic acid-based polymers for solid-phase extraction of deoxynivalenol and application to pasta analysis[J].Analytica Chimica Acta2008, 609:131-138.
[33]	何永红, 高志贤, 晁福寰.分子印迹-仿生传感器的研究进展[J].分析化学, 2004, 32 (10) :1407-1412.
[34]	YAQUB S, LATIF U, DICKERT F L.Plastic antibodies as chemical sensor materials for atrazine detection[J].Sensors and Actuators B:Chemical, 2011, 160 (1) :227-233.
[35]	RAI M, VARMA A.Mycotoxins in food, feed and bioweapons[M].Verlag Berlin Heidelberge:Springer, 2010:3-20.
[36]	BALAZS E, SCHEPERS J S.The mycotoxin threat to food safety[J].International Journal of Food Microbiolology, 2007, 119 (1-2) :1-2.
[37]	THIRUMALA-DEVI K, MILLER J S, REDDY G, et al.Phage-displayed peptides that mimic aflatoxin b1in serological reactivity[J].Journal of Applied Microbiology, 2001, 90 (3) :330-336.
[38]	LAI Weihua, FUNG D Y C, XU Yang, et al.Development of a colloidal gold strip for rapid detection of ochratoxin a with mimotope peptide[J].Food Control, 2009, 20 (9) :791-795.
[39]	YUAN Q, PESTKA J J, BRANDON M, et al.Identification of mimotope peptides which bind to the mycotoxin deoxynivalenol-specific monoclonal antibody[J].Appl Environ Microbiol, 1999, 65 (8) :3279-3286.
[40]	LIU Renrong, YU Zhou, HE Qinghua, et al.An immunoassay for ochratoxin a without the mycotoxin[J].Food Control, 2007, 18 (7) :872-877.
[41]	HE Qinghua, XU Yang, HUANG Yunhong, et al.Phage-displayed peptides that mimic zearalenone and its application in immunoassay[J].Food Chemistry, 2011, 126 (3) :1312-1315.
[42]	刘媛, 梁颖, 王耘, 等.抗独特型抗体在小分子农药、真菌毒素免疫检测中的应用[J].浙江农业学报, 2010, 22 (3) :398-402.
[43]	HE Jiang, FAN Mingtao, LIANG Ying, et al.Application of anti-idiotype antibody in small molecules immunoassay[J].Chin J Anal Chem, 2010, 38 (9) :1366-1370.
[44]	CHU F S, HUANG X, MARAGOS C M.Production and characterization of anti-idiotype and anti-anti-idiotype antibodies against fumonisin b1[J].Agric.Food Chem, 1995, 43:261-267.
[45]	江涛, 郑佳, 李楠, 等.抗T-2毒素单抗独特型抗体的制备及应用研究[J].中国食品卫生杂志, 2007, 19 (3) :234-237.
[46]	柳桢.黄曲霉毒素B-1免疫检测标准品替代研究[D].河南:郑州大学, 2006.
[47]	林超, 王东旭, 任洪林, 等.抗大田软海绵酸单抗独特型抗体的制备及鉴定[J].食品科学, 2011, 32 (11) :110-113.

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文章访问数: 95
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真菌毒素免疫分析中新型检测元件的研究进展

计量

出版历程

Research progress in novel materials for mycotoxin immunoassay

计量

出版历程

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