响应面分析优化酶法提取南瓜籽油的工艺研究

详细信息

Optimization of emzyme-assisted extraction processing of oil from pumpkin seed by response surface methodology

摘要: 采用纤维素酶辅助提取南瓜籽油,在单因素实验的基础上,利用Box-Benhnken的中心组合设计原理及响应面分析法,建立用酶量、反应时间、反应温度与提取率之间的数学模式,确定酶法提取南瓜籽油的较佳工艺为用酶量17mg/g,酶解时间为2.64h,酶解温度为47℃。南瓜籽油提取率达89.12%。
- 南瓜籽油; /
- 纤维素酶; /
- 提取率; /
- 响应面法;
Abstract: The cellulase-assisted extraction processing of oil from pumpkin seed was studied.On the basis of single -factor, the extraction conditions of oil from pumpkin seed by cellulase was optimized through Box-Benhnken design and response surface methodology.The mathematical regress model was established about extraction rate and enzyme dosage, time and temperature. According to this model, the optimum extraction conditions were determined as enzyme dosage 17mg /g, time 2.64h and temperature 47℃ .The extraction rate for this optimum condition was 89.12%.
- pumpkin seed oil;cellulase;extraction rate;response surface methodology; /
- undefined /
- undefined /
- undefined

[1]	魏冰.南瓜籽油的开发和利用研究[J].粮油加工, 2008 (5) :60-62.
[2]	董国玲, 田密霞, 姜爱丽, 等.南瓜籽油的开发利用价值[J].粮食科技与经济, 2010 (7) :33-35.
[3]	杨建新, 宋琳琳.南瓜籽油的萃取工艺及其性质研究[J].食品工业科技, 2010 (1) :263-264.
[4]	牛广才, 朱丹, 孙清瑞, 等.南瓜籽油超临界CO2流体萃取及其脂肪酸成分分析[J].中国粮油学报, 2010 (4) :31-33.
[5]	陈钊, 赵敏生, 白小芳, 等.南瓜籽油的冷榨制取研究[J].食品科技, 2005 (8) :88-90.
[6]	王庆铃, 张磊, 姬华, 等.水酶法提取南瓜子油工艺研究[J].粮食与油脂, 2011 (8) :21-23.
[7]	王小溪, 陈贵林.超声波提取南瓜籽油的工艺条件优化研究[J].食品科学, 2009 (8) :60-63.
[8]	高璐, 胡博然, 祁妤琳, 等.葡萄籽油提取工艺研究及对其理化性质的影响[J].食品科学, 2009 (22) :81-83.
[9]	李桂华.粮油油脂分析与检测[M].北京:化学工业出版社, 2006.
[10]	苗利利, 夏德水, 高丽娜.水酶法提取石榴籽油工艺研究[J].食品工业科技, 2010, 30 (12) :265-268.
[11]	张郁松, 寇炜材, 贾颖周.水酶法提取苹果籽油的工艺研究[J].粮油加工, 2007 (4) :49-51.
[12]	崔宝贵, 李珂.我国玉米胚芽制油的现状和发展趋势[J].农业机械, 2010 (9) :65-67.