响应面法优化Enterobacter sp.SYA2乳糖酶发酵工艺
详细信息Optimization of fermentation process for lactase by Enterobacter sp.SYA2 using response surface methodology
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摘要: 采用单因素实验对Enterobacter sp.SYA2产乳糖酶条件进行优化,优化后的发酵条件为:培养温度37℃、装液量50mL/250mL、摇床转速175r/min、接种量5%（V/V）、种龄8h,此条件下酶活为9.02U/mL;通过Plackett-Burman设计和Box-Behnken设计对菌株发酵培养基进行了优化,得到的最佳发酵培养基配方为:乳糖31.1g/L、蛋白胨26.0g/L、酵母膏5.0g/L、K2HPO43.0g/L、NaCl6.0g/L、MnCl20.5g/L、pH6.5,优化后的酶活为15.95U/mL。
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关键词:
- Enterobacter sp.SYA2; /
- 乳糖酶; /
- 优化; /
- 发酵工艺;
Abstract: Firstly, fermentation conditions for lactase by Enterobacter sp.SYA2 were optimized by single factor experiment, the result showed that the optimum temperature was 37℃, liquid volume in flask was 50mL /250mL, vorotation speed was 175r /min, inoculation volume was 5% ( V/V) , seed age was 8 hours.Then Plackett- Burman design and Box-Behnken Design were combined to optimize the medium components of lactase production.The results showed that the optimal medium were as follows:lactose 31.1g /L, peptone 26.0g /L, yeast extract was 5.0g /L, K 2 HPO 4 3.0g /L, NaCl 6.0g /L, MnCl 2 0.5g /L, initiation pH was 6.5. After optimization, the lipase activity was 15.95U /mL. -
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