响应面法优化微波辅助提取芋头多糖工艺研究
详细信息Study on the optimization of microwave-assisted extraction of polysaccharides from taro by response surface methodology
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摘要: 采用微波辅助水浴法提取芋头多糖并对工艺进行优化。在单因素实验的基础上,确定水提时间、水提温度、微波功率、微波处理时间4个因素的Box-Benhnken实验设计,以多糖的提取率为指标,采用响应面法优化芋头多糖的提取工艺,建立并分析各因素与指标的数学模型。结果表明:多糖提取的最佳工艺为水提时间为3h、水提温度为82℃、微波功率为395W、微波时间为77s,此时多糖的实际提取率为5.57%,与理论值之间的相对误差小于0.5%。说明通过响应面优化后得出的回归方程具有一定的实践指导意义。Abstract: The aim at developed an optimal procedure for the microwave- assisted extraction of polysaccharides from taro.Based on the single factor experimental results, Box- Benhnken experimental design was used to select the most important ones affecting extraction of taro polysaccharides, the optimal values of selected parameters, namely water extraction time, water extraction temperature, microwave power and microwave treatment time. Response surface experiments were used to optimize the extraction efficiency of a mathematic model between the factors and the index was established.Results showed that the optimal extraction parameters were water extraction time of 3h, water extraction temperature of 82℃, microwave power of 395W and microwave treatment time of 77s. The actual polysaccharides yield was 5.57% , compared with the theoretical value the relative error was less than 0.5%. The regression equation which was optimized by response surface method had a certain practical significance.
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