薄荷不同溶剂提取物抗氧化活性的研究
详细信息Study on the antioxidant activity of different extracts from Mentha canadensis L.
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摘要: 采用1,1-二苯基-2-三硝基苯肼（DPPH）、2,2’-联氮基双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）、Fe+还原法（FRAP）三种抗氧化模型对薄荷的水、甲醇、70%乙醇、正丁醇、乙酸乙酯五种提取物进行抗氧化活性评价,同时分析抗氧化活性与总酚和总黄酮含量的关系。结果表明,薄荷不同提取物均表现出良好的抗氧化活性,其抗氧化活性与多酚含量呈极显著相关,与黄酮含量相关性较小。其中,水提物对DPPH自由基清除率最高,EC50值为（0.53±0.04）mg/mL;甲醇提取物对ABTS自由基清除率最高,EC50值为（1.57±0.03）mg/mL;乙酸乙酯提取物的FRAP值最高为（4.73±0.03）mmol/g;水提物中总酚含量最高为（58.81±3.10）mg/g,甲醇提取物中总黄酮含量较高为（162.95±1.91）mg/g。Abstract: Five different extracts of Mentha canadensis L. through water, ethanol, 70% ethanol, butanol and ethyl acetate were evaluated by tree models of antioxidant activity:1, 1- diphenyl-2- picrylhydrazyl ( DPPH) , 2, 2'- azino -bis ( 3- ethylbenzthiazolne- 6-sulfonic acid ( ABTS) and ferric reducing antioxidant potential assay ( FRAP) . The relationship between the antioxidant activity and the content of total phenolics and total flavonoids was analysed. The results showed that all the extracts had great antioxidant activity and the content of total phenolics had a highly significant correlation with the activity, however, low correlation with the content of total flavonoids.The water extract showed that the highest radical scavenging rate on DPPH and the EC 50 was ( 0.53 ± 0.04) mg /mL. The methanol extract showed the highest radical scavenging rate on ABTS and the EC 50 was ( 1.57 ± 0.03 ) mg /mL. The ethyl acetate extract showed the highest value of FRAP and EC 50 was ( 4.73 ± 0.03) mmol /g.The highest content of total phenolics and total flavonoids came from water extract and methanol extract and the content of them were ( 58.81 ± 3.10) , ( 162.95 ± 1.91) mg /g, respectively.
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