高效液相色谱-蒸发光散射测定瓜蒌中L-瓜氨酸的含量
详细信息Separation and identification of L-citrulline in Trichosanthis Fructus by high performance liquid chromatography-evaporative light scattering detection
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摘要: 建立了高效液相色谱-蒸发光散射测定瓜蒌中L-瓜氨酸含量的实验方法。色谱分离使用Agilent ZORBAXSB-Aq柱（250mm×4.6mmi.d.,5μm）,流动相为水,流速为1.0mL/min。柱后流出物采用蒸发光散射检测器检测,飘移管温度为115℃,空气做载气,流速为3.2L/min。L-瓜氨酸的峰面积的对数值与质量浓度的对数值呈良好的线性关系,线性范围为0.05～0.75mg/mL,检出限为12.9μg/mL,加标回收率为98.36%,相对标准偏差为1.77%。将该法用于实际样品的测定,结果表明瓜蒌中L-瓜氨酸的含量为0.1mg/g左右。该方法简单、快速、准确、重现性好,适用于瓜蒌药材中L-瓜氨酸的定量分析。Abstract: A high performance liquid chromatography- evaporative light scattering detection method for separation and determination of L-citrulline in Trichosanthis Fructus was established. The analytical column was Agilent ZORBAX SB-Aq ( 250mm × 4.6mm i.d., 5 μm) .The mobile phase was water, and the flow rate was 1.0mL /min.The sample was determined by an evaporative light scattering detector.The temperature of drift tube was kept at 115℃ and the flow rate of atmosphere was 3.2L /min.The result showed that under the optimized conditions, the logarithm correlation between peak area and the concentration of L-citrulline was in a good linearity. The calibration curve had good linearity in the range of 0.05 ～ 0.75mg /mL, the detection limit of L-citrulline was 12.9 μg /mL, and the average recovery was 98.36% ( n = 5, RSD = 1.77% ) . The method was applied in the detection of L-citrulline in Trichosanthis Fructus, and the result revealed that the content was about 0.1mg /g. The method is a convenient, rapid and effective method for qualitatively analysis of L-citrulline in Trichosanthis Fructus.
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