响应面法优化马血中SOD超声波提取工艺研究

基金项目:

国家自然科学基金资助项目(21245003)；

详细信息

Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology

摘要: 以SOD比活力为响应值,在单因素实验的基础上,筛选出超声时间、超声功率、超声温度三个对提取影响较大的因素,利用响应面法进行优化,并验证优化方案。结果表明,SOD超声波提取的优化参数为:超声时间15min、超声功率220W、超声温度31℃。在此条件下,SOD比活力为77.5U/mg,与模型高度拟合。
- 马血; /
- SOD; /
- 超声波提取; /
- 响应面法;
Abstract: Based on single factor experiment, three main factors were creened out, including the ultrasonic time, ultrasonic power, ultrasonic temperature. Optimization of these factors by using response surface methodology was studied. Results of response surface analysis showed that the optimum ultrasonic extraction conditions of SOD were as follows : ultrasonic time 15min , ultrasonic power 220W , the ultrasonic temperature 31 ℃ . Under these conditions, specific activity of SOD was 77.5U/mg and which was in good agreement with the predicted value.
- horse blood;SOD;ultrasonic extraction;response surface methodology; /
- undefined /
- undefined /
- undefined

[1]	张良, 薛刚, 张彩莹, 等.牛血铜锌超氧化物歧化酶分离提取新工艺[J].食品科学, 2004, 25 (8) :121-123.
[2]	徐桂花, 于颖.羊血中超氧化物岐化酶 (SOD) 活性的测定[J].肉类工业, 2009 (1) :31-33.
[3]	于颖, 王小丽, 徐桂花.响应面分析法优化羊血中SOD的热变性提纯工艺条件[J].肉类研究, 2009 (11) :25-30.
[4]	邱玉华, 杨艳芳, 何颖, 等.等电点沉淀一超滤提取猪血SOD[J].科技资讯, 2008, 14:23.
[5]	金应世, 金晓玲.从动物血液提取SOD的工艺流程及技术[J].中国资源综合利用, 2000 (11) :13-14.
[6]	韩国才.我国马业的新趋势[J].中国动物保健, 2005 (10) :26.
[7]	邓莉, 刘章武, 杜金平.响应面法优化鹅血中SOD热变性提纯工艺条件[J].食品工业科技, 2011 (7) :270-273.
[8]	乌云达来, 孙振钧, 王冲, 等.牛血铜锌超氧化物岐化酶规模化生产工艺研究[J].家畜生态学报, 2006, 27 (5) :46-52.
[9]	许雅娟, 赵艳景, 胡虹, 等.邻苯三酚自氧化法测定超氧化物歧化酶活性的研究[J].西南民族大学学报, 2006, 32 (6) :1207-1209.
[10]	Douglas C Montgomery, 等.Design and Analysis of Experiments[M].傅珏生译.6th Edition.北京:人民邮电出版社, 2009, 347-389.