Application of luminescent Vibrio fischeri to evaluation of toxicity of several common pigments
-
摘要: 在探寻出色素与费氏弧菌发光抑制率存在相关性的基础上,初步建立起评价色素生物毒性的方法,并将其应用于果汁中色素产生的综合毒性的检验。结果:色素溶液质量浓度与发光抑制率呈正相关,相关系数在0.8760～0.9873之间,EC50范围在0.72～2.86mg/L之间;果汁中色素质量浓度与发光抑制率同样呈正相关,相关系数在0.9136～0.9951之间,EC50值在1.02～2.96mg/L之间。柠檬黄毒性较小,苋菜红毒性较大。果汁对人工合成色素的生物毒性具有一定影响。色素在果汁中对费氏弧菌发光抑制率随浓度增加而上升的幅度明显大于在水溶液中。本研究为食品急性生物毒性监测的研究提供初步参考。Abstract: A method built on the basis of the toxicity effect of pigments on luminescent bacterium Vibrio fisheri was developed for determining the toxicity and content of pigments. Results showed that there was a positive correlation between inhibitory rate of pigments against luminescent intensity of Vibrio fisheri and pigments concentration in aqueous solution, with a correlation coefficient ranging within 0.87600.9873. EC 50 range was 0.72 2.86mg/L. Inhibitory rate of pigments against luminescent intensity of Vibrio fisheri was also positively correlated with positive concentration in fruit juice, and the correlation coefficient was between 0.9136 and 0.9951. EC 50 range was 1.022.96mg/L. This showed that the toxicity of tartrazine was lower and the toxicity of amaranth was larger. Fruit juice played a role of mitigating the toxicity of the synthetic pigments. The bioluminescence inhibition rate by the pigments in the fruit juice increased with the rate significantly was higher than in aqueous solution. The study provided the initial reference for the acute toxicity testing of the food.
-
Keywords:
- Vibrio fisheri;pigment;toxicity; /
-
[1] 韦东普, 马义兵, 陈世宝.应用淡水发光细菌测定土壤Cu急性毒性的影响因素研究[J].农业环境科学学报, 2009 (7) :1386-1391. [2] Shahid Parvez, Chandra Venkataraman, Suparna Mukherji.Toxicity assessment of organic pollutants:Reliability of bioluminescence inhibition assay and univariate QSAR models using freshly prepared Vibrio fischeri[J].Toxicology in Vitro, 2008, 22 (7) :1806-1813.
[3] K Froehner, T Backhaus, LH Grimme.Bioassays with Vibrio fischeri for the assessment of delayed toxicity[J].Chemosphere, 2000, 40 (8) :821-828.
[4] T Backhaus, M Scholze, LH Grimme.Vibrio fischeri[J].Aquatic Toxicology, 2000, 49 (1/2) :49-61.
[5] Javier Bayo, JoséM Angosto, M Dolores Gómez-López.Ecotoxicological screening of reclaimed disinfected wastewater by Vibrio fischeri bioassay after a chlorination-dechlorination process[J].Journal of Hazardous Materials, 2009, 172 (1) :166-171.
[6] Antje Brack, Jürgen Strube, Peter Stolz, et al.Vibrio fischeri[J].Biochimica et Biophysica Acta (BBA) -General Subjects, 2003, 1621 (3) :253-260.
[7] E Fulladosa, JC Murat, M Martínez, et al.Patterns of metals and arsenic poisoning in Vibrio fischeri bacteria[J].Chemosphere, 2005, 60 (1) :43-48.
[8] 张国辉, 赵吉, 邵玉琴, 等.发光弧菌快速检测液态奶中的三聚氰胺[J].食品科学, 2010, 31 (6) :145-147. [9] 中华人民共和国国家标准.GB2760-2007食品添加剂使用卫生标准[S].中国标准出版社, 2007. [10] 蒋利刚, 程东, 韩晓莹, 等.柠檬黄对雄性小鼠生殖细胞的影响[J].生物医学工程研究, 2011, 30 (3) :174-176. [11] 杨国先.饮料中合成色素的方法研究[J].食品工业科技, 2002, 23 (3) :66-67. [12] 李帮锐, 冯家力, 潘振球, 等.高效液相色谱-质谱/质谱联用法测定饮料中的人工合成色素[J].中国卫生检验杂志, 2007, 17 (4) :579-585. [13] 陈晓红, 李小平, 姚浔平.高效液相色谱-质谱联用法测定饮料中人工合成色素的研究[J].中国卫生检验杂志, 2005, 15 (8) :941-942. [14] 皇甫鑫, 廖翀, 杨坪, 等.金属化合物对发光菌的毒性效应及不同发光菌的敏感度差异研究[J].环境科学学报, 2010, 30 (9) :1787-1792.
计量
- 文章访问数: 189
- HTML全文浏览量: 9
- PDF下载量: 300
下载:
