响应面分析法优化乳白香青中绿原酸提取工艺
详细信息Optimization of extraction technology of chlorogenic acid from Anaphalis lactea Maxim. by response surface methodology
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摘要: 以乳白香青为原料,确定乳白香青中绿原酸最佳提取的工艺条件。在单因素实验的基础上,运用Box-Behnken中心组合实验设计,建立绿原酸提取得率的二次回归模型,确定乳白香青中绿原酸的优化工艺条件为:提取温度为41℃,液料比为31∶1（mL/g）,甲醇浓度为66%（v/v）,绿原酸的得率为5.2796（mg/g）,接近于模型预测值5.3295（mg/g）。Abstract: In order to promote the extractability of chlorogenic acid from Anaphalis lactea Maxim, we established a quadric regression equation for predicting the yield of chlorogenic acid on the basis of one-factor tests, according to Box-Behnken design . The result showed that the optimum conditions for the extraction of chlorogenic acid were as follows:extraction temperature 41℃ , ratio of material to solvent 31∶1 (mL/g) , Mthanol concentration 66% (v/v) . Under these conditions, the chlorogenic acid yield was up to 5.2796 (mg/g) which was well matched with the predictive extracting yield 5.3295 (mg/g) .
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