Abstract: In order to promote the extractability of chlorogenic acid from Anaphalis lactea Maxim, we established a quadric regression equation for predicting the yield of chlorogenic acid on the basis of one-factor tests, according to Box-Behnken design . The result showed that the optimum conditions for the extraction of chlorogenic acid were as follows:extraction temperature 41℃ , ratio of material to solvent 31∶1 (mL/g) , Mthanol concentration 66% (v/v) . Under these conditions, the chlorogenic acid yield was up to 5.2796 (mg/g) which was well matched with the predictive extracting yield 5.3295 (mg/g) .