响应面法优化深黄被孢霉发酵生产花生四烯酸的培养基条件

Optimization of Mortierella Isabellina culture medium conditions in fermentation of arachidonic acid by response surface methodology

摘要: 为了降低深黄被孢霉YZ-124生产花生四烯酸的成本,研究了不同添加量的玉米黄浆水对发酵的影响,与葡萄糖培养基相比,在发酵培养基中添加一定量的玉米黄浆水对发酵产量无显著影响。在单因素实验的基础上,利用Design Expert设计了响应面实验,研究了葡萄糖浓度、不同添加量的玉米黄浆水和初始pH对花生四烯酸产量的影响。结果表明,最佳的培养基条件是葡萄糖浓度为90g/L、添加体积分数为25%的玉米黄浆水、初始pH6时,花生四烯酸（ARA）产量达到最大,为3.11g/L。

Abstract: Fermentative production arachidonic acid of Mortierella isabellina using waste water from maize paste was investigated to reduce the cost, adding waste water from maize paste into the culture medium had no obvious effect on the fermentation. On the basis of single factor tests, response surface analysis was designed by Design Expert, and the glucose concentration, different quality of waste water from maize paste and pH were investigated. The results showed that the optimal culture medium conditions were the glucose concentration was 90g/L, the maize paste waste water was 25% in the culture medium and pH was 6. Under this condition, the yield of arachidonic acid was 3.11g/L.