纤维素酶提取冬枣叶中总黄酮工艺的研究

Study on extraction technology of total flavones using cellulase from leaves of winter-jujube

摘要: 采用纤维素酶提取冬枣叶中的总黄酮。以芦丁为标准品,采用分光光度法测定冬枣叶中总黄酮的含量,通过单因素实验研究提取温度、提取时间、酶用量和料液比对提取率的影响,再利用正交实验优化最佳提取工艺条件。结果表明,纤维素酶提取冬枣叶中总黄酮的最佳工艺条件为:料液比1:50(g/mL),提取温度55℃,提取时间90min,酶用量4mg/g。在最佳提取工艺条件下,提取率可达2.45%。同时得到各影响因素对总黄酮提取率的显著性影响顺序为:提取温度>提取时间>酶用量>料液比。

Abstract: Total flavones in leaves of winter-jujube were extracted using cellulose.The total flavones extraction rate was determined by spectrophotometry method with rutin as reference substance.In the single factor analysis, the effect of extraction temperature, extraction time, enzyme amount and solid-liquid ratio were measured.The orthogonal test was used to optimize the conditions of extraction.The results showed that the best conditions of extraction total flavones from leaves of winter-jujube were the solid-liquid ratio 1:50 (g/mL) , extraction temperature 55℃, extraction time 90min, enzyme amount 4mg/g.Under this optimal condition, extraction rate was 2.45%.The results showed that the order of extraction rate of total flavones was as follows:extraction temperature>extraction time>enzyme amount>solid-liquid ratio.