柱后补偿液相色谱与电雾式检测器结合测定茶氨酸含量
详细信息Determination of theanine in tea-extraction by HPLC with a compensate after column and charged aerosol detection
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摘要: 建立液相色谱柱后补偿与电雾式检测器（CAD）结合的方法测定了茶叶粗提物中的茶氨酸。结果表明:茶氨酸线性回归方程为Y=0.1412X-0.0614,相关系数r=0.9998,线性范围为1.50～50.00μg/mL,方法最低检出限为0.50μg/mL（以S/N=3计）,定量限为1.50μg/mL（以S/N=10计）;空白样品加标回收率为97.62%～112.13%;重现性实验表明（n=5）,茶氨酸的保留时间差值小于0.2min,峰面积的相对标准偏差（RSD值）为0.62%,重现性较好。该方法重现性好、灵敏度高,操作简单,适用于茶叶提取物中茶氨酸的检测。Abstract: An analytical method for the determination of theanine in tea-extraction by HPLC with compensate after column and charged aerosol detector (CAD) was developed.The results showed that the calibration formula was Y=0.1412X-0.0614, the correlation coefficient was above 0.9998, and the calibration curve was linear in the range of 1.50 ~ 50.0μg/mL, and the detection limit of the method (calculate with S/N=3) was 0.50μg/mL, and limit of quantitation was 1.50μg/mL (calculate with S/N=10) .Then a sample was analyzed with this method, and the recoveries of blank sample spiked were 97.62% ~ 112.13%, and the difference of retention time was less than 0.2min and the relative standard deviation of peak area was 0.62% (n=5) .The sensitive and accurate method was successfully applied to the analysis of theanine in tea-extraction.
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