桉叶脂肪酸提取成分的GC-MS优化分析
详细信息Optimization of GC-MS conditions for fatty acid composition in Eucalyptus leaves using D-optimal response surface methodology
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摘要: 以华南广泛栽培的尾巨桉DH32-29无性系为材料,采用D-optimal响应面方法对桉叶脂肪酸组成成分检测的GC-MS色谱条件进行优化,探讨了色谱条件的程序升温中的8个因子对桉叶提取物脂肪酸成分的影响。以脂肪酸成分的相对含量、脂肪酸种类数、Simpson指数、离子谱图质量为响应值,优化桉叶提取物脂肪酸成分得到的色谱条件为:初始温度80℃,第一次保留时间1.1min,升温速率1为17℃/min,中间温度240℃,第二次保留时间0.7min,升温速率2为1℃/min,最后温度253℃,最后保留时间4min。分别以优化前色谱条件和优化后色谱条件对16个不同提取方法获得WC3桉叶提取物进行对比,结果表明后者在桉叶脂肪酸含量、成分种类数、脂肪酸成分的Simpson指数上显著优于前者。Abstract: The fatty acids in Eucalyptus urophylla×E. grandis (DH32-29 clone) leaves were extracted by ultrasonic extraction method, and its compositions were analyzed by GC-MS. The D-Optimal response surface methodology were used to optimize eight factors of GC-MS conditions. The content, kinds, Simpson index and TIC of fatty acids were used as responsed value to evaluate the effectiveness of treatment factors. The results of the optimal conditions were as follows:initial temperature 80℃, initial first time (1. 1min) , temperature rate 1∶ 17℃/min) , hold temperature 240℃, hold second time 0. 7min, temperature rate 2∶ 1℃/min, final temperature 253℃, final hold time 4min. Compared with extraction of WC3 leaves from 16 different extraction treatments, the results were also showed that the optimum GC-MS conditions were better than the normal conditions obviously in the content, kinds, Simpson index and TIC of fatty acids.
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[1] 祁述雄.中国桉树[M].北京:中国林业出版社, 2002. [2] 国家药典委员会.中华人民共和国药典 (一部) [M].北京:化学工业出版社, 2005. [3] 梁振益, 杨雪蕊, 李海峰, 等.桉树叶中黄酮类化合物的提取工艺研究[J].海南大学学报:自然科学版, 2007 (3) :256-258. [4] 马自超, 劳伦斯·波特.桉树叶单宁组分的研究[J].植物学报:英文版, 1988, 30 (5) :534-538. [5] Hou A J, LYZ, Yang H, et al.Hydrolyzable Tannins and related polyohenols from eucalyptus globulus[J].Journal of Asian Natural Products Research, 2000 (3) :205-212.
[6] 孙汉洲, 赵芳, 李志辉.用细胞膜脂脂肪酸成分分析法筛选抗寒巨桉种源[J].中南林学院学报, 2000, 20 (3) :59-62. [7] 李志辉, 汤珧华, 孙汉洲, 等.耐寒性桉树早期选择Ⅲ巨桉种源和家系膜脂肪酸组成、含量与抗寒性关系[J].中南林学院学报, 2000, 20 (3) :80-85. [8] Guimaraes R BL, Carvalho A M, et al.Aromatic plants as a source of important phytochemicals:vitamins, sugars and fatty acids in cistus ladanifer, cupressus lusitanica and eucalyptus gunnii leaves[J].Industrial Crops and Products, 2009, 30 (3) :427-430.
[9] 惠尉, 陈国广, 唐俊, 等.响应面分析法优化盐酸丁螺环酮微球制备工艺研究[J].时珍国医国药, 2008, 19 (9) :2268-2271. [10] R V Muralidhar RRC, R Marchant P Nigam.A response surface approach for the comparison of lipase production by Candida Cylindracea using two different carbon sources[J].Biochemical Engineering Journal, 2001, 9 (1) :17-23.
[11] 张婧, 谢耀坚, 杜阿朋, 等.尾叶桉叶片脂肪酸成分的GC-MS分析[J].桉树科技, 2011, 28 (1) :9-13. [12] 寇秀颖, 于国萍.脂肪和脂肪酸甲酯化方法的研究[J].食品研究与开发, 2005, 4 (2) :46-47. [13] Piepel GF.Programs for generating extreme vertices and centroids of linearly constrained experimental regions[J].Journal of Quality Technology, 1988, 20 (2) :125-139.
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