响应面法优化竹叶黄酮萃取工艺及其抗氧化研究
详细信息Study on optimization of extraction of falconoid from bamboo leaves by response surface analysis and its antioxidant activity
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摘要: 利用微波提取慈竹叶中总黄酮类化合物。在单因素实验的基础上,采用响应面法研究微波提取的最佳工艺条件。结果表明,以80%的乙醇为提取溶剂,微波提取最佳条件为:提取温度70℃、提取时间10.5min、液固比为34∶1mL/g;在此工艺条件下,慈竹叶总黄酮得率为1.381%。抗氧化活性研究表明,慈竹竹叶总黄酮DPPH·清除作用较好,其IC50值为141.5mg/L,优于相同浓度的2,6-二叔丁基对甲酚（BHT）。Abstract: The extraction of total flavoniods from Neosinocalamus affinins leaves by microwave-assisted extraction was carried out, and the optimal extraction conditions was studied, such as extraction temperature, extraction time and liquid-to-solid ratio, by response surface analysis (RSA) based on single factor tests. The results showed that the optimal extraction conditions were as follows in the presence of 80% ethanol as solvent, extraction temperature 70℃, extraction time 10. 5minutes and liquid-to-solid ratio 34mL/g. Under the optimal conditions, the yield of total flavoniods from Neosinocalamus affinins leaves reached 1. 381%. Antioxidant activities were evaluated by using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical in vitro. The IC50 was 141. 5mg/L, and the result showed that the DPPH radical scavenging activity of flavonoids was stronger that of DPPH.
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