响应面法优化蜂胶黄酮提取工艺及其抗氧化活性研究
详细信息Optimization of extraction process of propolis flavonoids by response surface methodology and its antioxidant activity research
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摘要: 采用响应面法对蜂胶黄酮提取工艺进行优化。以蜂胶为原料,基于单因素实验,以乙醇体积分数、液料比、提取时间和提取温度为相应因素、蜂胶黄酮提取率为响应值,采用四因素三水平的响应面分析法,确定最佳提取工艺条件为:液料比14:1（mL·g-1）、提取温度72.6℃、提取时间2.5h、乙醇浓度80%（v/v）,在此条件下理论提取率为33.9324%,与实测值基本相符,说明优化工艺可行。以VC为对照物,经DPPH法和铁氰化钾法验证蜂胶黄酮的抗氧化活性,发现蜂胶黄酮具有较强的清除DPPH自由基能力和还原能力,可作为优良的天然抗氧化剂资源。Abstract: Propolis flavonoids were extracted by ethanol, and the extraction process was optimized by response surface analysis.On the basis of one-factor-at-a-time experiment, optimal extraction conditions for maximizing popolis flavonoids were determined by using a 4-variable, 3-level Box-Behnken experimental design combined with response surface analysis as follows:the liquid-material ratio was 14:1 (mL·g-1) , the extraction temperature was 72.6℃, the extraction time was 2.5h, the ethanol concentration was 80% (v/v) , resulting in an propolis flavonoids yield of 33.82%, which was basic agreement with the predicted value of 33.9324%.Thus, the optimal process was reliable.The antioxidant activity in vitro condition of propolis flavonoids were evaluated by DPPH free radical scavenging assay and potassium ferricyanide reduction assay using V C as the control.Propolis flavones were found to have powerful DPPH free radical scavenging capacity and reducing power.Thus, propolis flavonoids could be developed as excellent natural antioxidant resources.
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