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中国精品科技期刊2020

水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法测定

水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法测定[J]. 食品工业科技, 2012, (20): 80-83. DOI: 10.13386/j.issn1002-0306.2012.20.017
引用本文: 水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法测定[J]. 食品工业科技, 2012, (20): 80-83. DOI: 10.13386/j.issn1002-0306.2012.20.017
Determination of residues of chloramphenicol, thiamphnicol and florfenicol in fishery products by HPLC-UVD[J]. Science and Technology of Food Industry, 2012, (20): 80-83. DOI: 10.13386/j.issn1002-0306.2012.20.017
Citation: Determination of residues of chloramphenicol, thiamphnicol and florfenicol in fishery products by HPLC-UVD[J]. Science and Technology of Food Industry, 2012, (20): 80-83. DOI: 10.13386/j.issn1002-0306.2012.20.017

水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法测定

基金项目: 

湛江市财政资金科技专项项目(2011D02);

详细信息
  • 中图分类号: TS254.7;O657.72

Determination of residues of chloramphenicol, thiamphnicol and florfenicol in fishery products by HPLC-UVD

  • 摘要: 建立同时检测水产品中氯霉素、甲砜霉素和氟苯尼考的HPLC-UVD法。以2%碱性乙酸乙酯作为提取溶剂,正己烷脱脂后直接经高效液相色谱进行分析。氯霉素、甲砜霉素和氟苯尼考在0.10~10.00mg/L范围内有良好的线性关系。在添加浓度为0.25~5.00mg/kg时,平均回收率在81.00%~102.93%之间,RSD在1.95%~7.10%之间。氯霉素的检出限为0.01mg/kg,甲砜霉素和氟苯尼考的检出限为0.02mg/kg。 
    Abstract: A high-performance liquid chromatography with ultraviolet detection method was developed for simultaneous determination of Chloramphenicol (CAP) , Thiamphnicol (TAP) and Florfenicol (FF) in fishery products. Chloramphenicols (CAPs) residues were extracted by basic ethyl acetate , followed by sample cleanup with n-hexane and quantization by liquid chromatography with ultraviolet detector. Good linearities were obtained in the concentration ranging from 0.10 ~10.00mg/L for CAP, TAP and FF. The average recoveries and relative standard deviations (RSDs) for the analysis of all samples fortified over the range of 0.25~5.00mg/kg were in the range of 81.00%~102.93% and 1.95%~7.10%, respectively. The limits of detection were 0.01mg/kg for CAP and 0.02mg/kg for TAP and FF.
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出版历程
  • 收稿日期:  2012-05-13

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