马铃薯多酚氧化酶酶学特性及热稳定性模型的研究
详细信息Enzymatic characteristics and thermal stability model of polyphenol oxidase from potato
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摘要: 目的:研究马铃薯中多酚氧化酶(polyphenol oxidase,PPO)的部分酶学性质及热稳定性并拟合数学模型预测酶活变化过程。方法:采用紫外可见分光光度计测定马铃薯多酚氧化酶活力,利用Matlab软件拟合二元多项式热稳定性数学模型,SPSS软件对多项式模型回归分析。结果:马铃薯多酚氧化酶的最适pH为5.5,最适反应温度为40℃,最适底物浓度为8.33mmol/L,加工温度、时间与酶活残存率的函数关系为:Z=24.5439+6.1163X+3.4064Y-0.0269X2-0.2361XY-0.0351Y2-0.0002836X3+0.001326X2Y+0.0012XY2,其中R2=0.981,F=100.695,p<0.001。结论:二元三次多项式模型能够较准确的反映并预测马铃薯多酚氧化酶在热加工过程中酶活的变化趋势。Abstract: Objective:Evaluated the enzymatic characteristics and thermal stability of polyphenol oxidase (PPO) from potato and fit stability model to predictive the activity of PPO in processing.Methods:Spectrophotometer method was applied to detect the enzyme activity.The thermal stability model of PPO was fitted with Matlab.The regression analysis of polynomial model was analyzed by SPSS.Results:The optimum temperature of polyphenol oxidase was 40℃ and pH was 5.5, substrate concentration was 8.33mmol/L.The relationship of technological parameter (time X, temperature Y) and residual activity of PPO was as following:Z=24.5439+6.1163X+3.4064Y-0.0269X2-0.2361XY-0.0351Y2-0.0002836X3+0.001326X2Y+0.0012XY2 with R2=0.981, F=100.695, p<0.001.Conclusion:The model was accurate and had ability of predicting the tendency of enzyme activity changing of PPO during processing.
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[1] 吴文标, 盛德贤, 吕世安.马铃薯酚类化合物的研究[J].中国马铃薯, 2001, 15 (3) :158-162. [2] Lewis C E, Walker J R L, Lancaster J E, et al.Determination of anthocyanins, flavonoids and phenolic Acids in potatoes.I:coloured cultivars of solanum tuberosum L[J].J Sci Food Agric, 1998, 77:45-57.
[3] Kamnerdpetch C, Weiss M, Kasper C, et al.An improvement of potato pulp protein hydrolyzation process by the combination of protease enzyme systems[J].Enzyme and Microbial Technology, 2007, 40:508-514.
[4] Sanchez-Ferrer A, Rodriguez-Lopez J N, Garcia-Canovas F, et al.Tyrosinase:a comprehensive review of its mechanism[J].BBA-protein and Molecular Enzymology, 1995, 1247 (1) :1-11.
[5] Wang C Y, Mellethin W M.Relationship of friction discoloration to phenolic compounds'd'Anjou'pears[J].Hortsci, 1973 (8) :321-323.
[6] 张莉, 陈乃富.苔干多酚氧化酶的酶学特性研究[J].食品工业科技, 2011, 32 (12) :200-202. [7] 李佩艳, 刘建学, 徐宝成, 等.不同抑制剂对澳洲青苹中多酚氧化酶活性的影响[J].中国食品添加剂, 2010 (1) :160-163. [8] 李瑜, 杨国浩, 詹丽娟, 等.双孢菇中多酚氧化酶活性的影响因素[J].食品科学, 2011, 32 (18) :319-322. [9] 张国庆, 董明, 李娜, 等.宣木瓜多酚氧化酶酶学特性与抑制剂研究[J].食品科学, 2011, 32 (10) :288-291. [10] Marri C, Frazzoli A, Hochkoeppler A, et al.Purification of a polyphenol oxidase isoform from potato (So-lanum tuberosum) tubers[J].Phytochemistry, 2003, 63 (7) :745-752.
[11] 姜绍通, 罗志刚, 潘丽军.甘薯中多酚氧化酶活性的测定及褐变控制[J].食品科学, 2001, 22 (3) :19-22. [12] Rompel A, Fischer H, Meiwes D, et al.Substrate specificity of catechol from Lycopus europaeus and characterization of bioproducts of enzymic caffeic acid oxidation[J].FEBS Letters, 1999, 445:103-110.
[13] Capitani C D, Carvalho A C L, Botelho P B.Synergism on antioxidant activity between natural compounds optimized by response surface methodology[J].Eur J Lipid Sci Technol, 2009, 111:1100-1110.
[14] Santos T B D, Mitchell D A, Krieger N, et al.Experimental and modelling study of enzymatic oxidation of5-o-Caffeoylquinic acid by polyphenol oxidases[J].Food Technol Biotechnol, 2010, 48 (4) :548-553.
[15] Noorizadeh H, Farmany A.Exploration of linear and nonlinear modeling techniques to predict of retention index of essential oils[J].Journal of the Chinese Chemical Society, 2010, 57:1268-1277.
[16] 李桂琴, 刘坤, 高志华, 等.鸭梨果肉多酚氧化酶的活性研究与纯化[J].河北农业大学学报, 2010, 33 (5) :45-49. [17] Gawlik-Dziki U, Zotek U, Swieca M.Characterization of polyphenol oxidase from butter lettuce (Lactuca sativa var.capitata L.) [J].Food Chemistry, 2008, 107 (1) :129-135.
[18] Buckow R, Weiss U, Knorr D.Inactivation kinetics of apple polyphenol oxidase in different pressure-temperature domains[J].Innovative Food Science&Emerging Technologies, 2009, 10 (4) :441-448.
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