Abstract: ObjectiveEstablished the method to determine the contents of 14 main compounds in natural apple essence using Head Space-Solid Phase Microextraction, and gas chromatography mass spectrometry.Method The optimal HS-SPME condition was obtained through single factor and surface response design experiments.Standard curve was established by internal standard method to determine compounds'contents.ResultThe optimal conditions for HS-SPME were 85μm PA fiber, 2mL natural apple essence, 40℃ water bath, 0.4g/mL NaCl, 30min of extracting time.Results of content determination of compounds experiments:the precision experiment results RSD was 1.20%～3.60%;the repeatability experiment results RSD was 3.17%～8.86%;the recovery of chemical standards was 93.44%～107.42%.ConclusionThe method of HS-SPME/GC-MS coupled with internal standard curve provides high accuracy, precision, repeatability, was effective for content determination for compounds in apple essence.