玉米淀粉酶脱支处理过程的优化研究
详细信息Optimization of enzyme debranching process of corn starch
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摘要: 为优化酶脱支水解过程,在单因素实验的基础上,采用响应曲面法对酶脱支水解的3个主要影响因素即:pH、温度、和酶添加量进行优化。建立了各因素与酶脱支水解产生还原力之间的数学模型。得出的最佳处理条件为:温度56℃、pH5.1、酶添加量28.92U/g。模型R2=0.9438,说明其拟合程度良好,可以用该模型优化酶脱支处理玉米淀粉的过程。Abstract: The process of enzyme debranching corn starch was investigated. Based on the single factor experiment, the effect of pH, temperature and enzyme concentration on the yield of reducing value were optimized with Response Surface Methodology (RSM) . A mathematical model was established between the studied factors and the responses. The optimal conditions were as follows:temperature 56℃, pH5.1, and enzyme concentration 28.92U/g. The R2 of 0.9438 means that the results were in good agreement with predicted values and the model was useful for optimizing the enzyme debranching process.
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