蜂蜜中羟甲基糠醛的近红外光谱快速测定方法
Rapid determination method of hydroxymethyl furfural in honey by near infrared spectroscopy technique
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摘要: 为实现蜂蜜中羟甲基糠醛的快速测定,利用近红外（NIR）光谱分析技术结合偏最小二乘法（PLS）建立了蜂蜜中羟甲基糠醛的定量分析模型,并进行了预测。通过光谱扫描,波数范围为77064009cm-1、一阶导数、norris derivative平滑及10个因子数进行光谱预处理,偏最小二乘法（PLS）,交叉验证。结果表明,羟甲基糠醛定量模型的交叉验证相关系数（Rcv2）=0.99620、交叉验证均方差（RMSECV）=2.40;预测相关系数（Rp2）=0.99874、预测均方差（RMSEP）=2.02;预测值与测定值之间无显著差异,该方法适用于蜂蜜中羟甲基糠醛的快速测定。Abstract: The quantitative analysis model of hydroxymethyl furfural (HMF) in honey was built based on near infrared (NIR) spectroscopy technique and partial least squares (PLS) to determine HMF quickly. NIR spectrumswhich wavelength coverage chose 77064009cm-1 were preprocessed with first derivate (SF) , Norris derivative smoothing and ten factors. The model was validated by cross-validation method. Results showed that Rcv2 was 0.99620, RMSECV was 2.40, Rp2 was 0.99874 and RMSEP was 2.02 in this model. NIR spectroscopy technique was applied to determine HMF quickly since there was no significant deviation between predictive values and determination values.
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