两株乳酸菌发酵上清液对E.coli作用分析及培养条件优化
详细信息Analysis and optimization of against E.coli by fermented supernatant from two Lactobacillus
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摘要: 以植物乳杆菌和保加利亚乳杆菌为研究对象,以大肠杆菌为指示菌,通过琼脂扩散法首先分析植物乳酸杆菌和保加利亚乳酸杆菌的发酵上清液对大肠杆菌的抑菌活性差异,然后再观察不同培养温度、葡萄糖浓度、pH和培养时间对植物乳杆菌发酵上清液抑菌效果的影响。结果显示,植物乳杆菌在35℃,8%葡萄糖,pH为9时,发酵上清液抑菌效果较好。经过正交优化实验得出最佳条件组合为:培养温度35℃,培养基pH7,葡萄糖浓度8%,培养时间36h,抑菌圈直径为(1.451±0.016)cm。研究还发现,保加利亚乳杆菌发酵上清液对大肠杆菌无抑制作用。Abstract: In this study, using E.coli as the indicator bacteria the antibacterial activity against E.coli of the fermented supernatant of Lactobacillus plantarum and Lactobacillus bulgaricus by the agar diffusion was investgated.Then, the effect of culture temperature, glucose concentration, pH and cultivating time on the antibacterial activity of the Lactobacillus plantarum were studied.The results showed that when Lactobacillus plantarum were incubated at 35℃, glucose concentration 8% and pH9, the antibacterial activity of its fermentation supernatant fluid was pretty good.Orthogonal test was used to analyze the optimum culture conditions for planting Latcobacillus.The optimum culture conditions for its antibacterial activity were estimated to be, cultivation temperature at 35℃, incubating for 36h, glucose concentration 8% and pH7, and the bacteriostatic circle was measured at (1.451±0.016) cm in diameter.In addition, the results of this study suggested that Lactobacillus bulgaricus had no bacteriostasis to E.coli.
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[1] Daeschel M A.Antimicrobial substances from lactic acid bacteria for use as food preservatives[J].Food Technol, 1989, 43 (1) :164-171.
[2] Deegan L H, Cotter P C, Hill C, et al.Bacteriocins:Biological tools for bio preservation and shelf life extension[J].International Dairy Journal, 2006, 16 (9) :1058-1071.
[3] Virginia S O, Aida A P, Maria E N.Characterization of a bacteriocin like substance produced by avaginal lactobacillus salivarius strain[J].Appl Environ Microbiol, 1999, 65 (12) :5631-5635.
[4] Brtz H, Sahl H G.New insights into the mechanism of action of lantibiotics diverse biological effects by binding to the samemolecular target[J].J Antimicrob Chemother, 2000, 46:1-6.
[5] Heerklotz H, Wieprecht T, Seelig J.Membrane perturbation by the lipopeptide surfactin and detergents as studied by deuterium NMR[J].J Phys Chem B, 2004, 108:4909-4915.
[6] Blondelle S E, Lohner K, Aguilar M I.Lipid induced conformation and lipid binding properties of cytolytic and antimicrobial peptides:determination and biological specificity[J].Biochim Biophys Acta, 1999, 1462:89-108.
[7] Powell J E, Witthuhn R C, Todorov S D, et al.Characterization of bacteriocin ST8KF produced by a kefir isolate Lactobacillus plantarum ST8KF[J].International Dairy Journal, 2007, 17:190-198.
[8] Todorov S D, Nyati H, Meincken M, et al.Partial characterization of bacteriocin AMA-K, produced by Lactobacillus plantarum AMA K isolated from naturally fermented milk from Zimbabwe[J].Food Control, 2007, 18:656-664.
[9] Collado M C, Gonzalez A, Gonzalez R, et al.Antimicrobialpeptides are amongthe antagonistic metabolites produced by Bifidobacterium against Helicobacter pylori[J].Int J Antimicrob Agents, 2005, 25:385-391.
[10] Nieto-Lozano J C, Reguera-Useros J I, Peláez-Martínez M C, et al.Effect of a bacteriocin produced by Pediococcus acidilactici against Listeria monocytogenes and Clostridium perfringens on Spanish raw meat[J].Meat Science, 2006, 72:57-61.
[11] Matsusaki H, Endo N, Sonomoto K, et al.Lantibiotic nisin Z fermentative production by Lactococcus lactis IO1:relationship between production of the lantibiotic and lactate and cell growth[J].Appl Microbio Biot, 1996, 45 (1) :36-40.
[12] Mulders J W, Boerrigter I J, Rollema H S, et al.Identification and characterization of the lantibiotic nisin Z, a natural nisin variant[J].Eur J Biochem, 1991, 201 (3) :581-584.
[13] 石金舟, 陈丽园, 张明.植物乳杆菌R260产细菌素发酵条件的研究[J].中国微生态学杂志, 2006, 18 (5) :341-342. [14] Delgado A, Arroyo López F N, Brito D, et al.Optimum bacteriocin production by Lactobacillus plantarum17.2b requires absence of NaCl and apparently follows a mixed metabolite kinetics[J].J Biotechnol, 2007, 130 (2) :193-201.
[15] Ogunbanwo S T, Sanni A I, Onilude A A.Influence of cultural conditions on the production of bacteriocin by Lactobacillus brevis OG1[J].Afr J Biotechnol, 2003, 2 (7) :179-184.
[16] Paynter M J, Brown K A, Hayasaka S S.Factors affecting the production of an antimicrobial agent plantaricin F, by Lactobacillus plantarum BF001[J].Lett Appl Microbiol, 1997, 24 (3) :159-165.
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