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中国精品科技期刊2020

荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究

荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究[J]. 食品工业科技, 2012, (15): 220-223. DOI: 10.13386/j.issn1002-0306.2012.15.027
引用本文: 荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究[J]. 食品工业科技, 2012, (15): 220-223. DOI: 10.13386/j.issn1002-0306.2012.15.027
Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity[J]. Science and Technology of Food Industry, 2012, (15): 220-223. DOI: 10.13386/j.issn1002-0306.2012.15.027
Citation: Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity[J]. Science and Technology of Food Industry, 2012, (15): 220-223. DOI: 10.13386/j.issn1002-0306.2012.15.027

荚果蕨中多糖的超声辅助提取及其DPPH自由基清除作用的研究

基金项目: 

国家“十二五”科技支撑计划课题(2011BAI06B05);

详细信息
  • 中图分类号: TQ461

Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity

  • 摘要: 以荚果蕨(Matteuccia struthiopteris(L.)Todaro)根状茎为材料,采用单因素实验和正交实验探讨超声辅助提取多糖的最佳工艺条件,应用1,1-二苯基苦基苯肼(DPPH)自由基清除法评价荚果蕨根状茎中多糖的抗氧化活性。实验结果表明,超声辅助提取该多糖的最佳工艺参数为:提取温度为80℃、料水比为1:50(g/mL)、超声时间为30min、超声功率为150W。在此条件下提取的荚果蕨根状茎、营养叶、孢子叶3个部位中粗多糖的提取率分别为9.67%、3.30%、3.43%。同时,荚果蕨根状茎中多糖有较强的抗氧化作用,对DPPH自由基半数抑制浓度(IC50)为68.68μg/mL。研究荚果蕨多糖的超声辅助提取工艺及抗氧化活性,对荚果蕨天然保健品的开发利用有重大指导意义。 
    Abstract: The condition for ultrasonic extraction of polysaccharides from Matteuccia struthiopteris were optimized by one-factor-at-a-time and orthogonal array design methods.Polysaccharide from Matteuccia struthiopteris was tested with DPPH· to estimate their antioxidative activity.The results showed that the best extraction conditions were determined as below:ethanol liquid ratio was 1:50 (g/mL) , extraction temperature was 80℃, ultrasonic power was 150W, ultrasonic time was 30min.The extraction yield of polysaccharide in rhizome, trophophyll and sporophyl was 9.67%, 3.30% and 3.43% respectively.The polysaccharide had high antioxidative activity, revealing an IC50 of 68.68μg/mL.Investigating ultrasonic traction of polysaccharides from matteuccia struthiopteris and their DPPH free radical scavenging activity, it is a great direction significance in the development and utilization of matteuccia struthiopteris.
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出版历程
  • 收稿日期:  2012-02-15

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