Effects of Yeast-lactic Acid Bacteria Co-fermentation on the Quality of Navel Orange Beverage

YE Hong; ZHOU Xuerui; WANG Su; ZHANG Yingyue; SUN Yue; ZENG Chaoyi; ZHANG Qing; TANG Jie

doi:10.13386/j.issn1002-0306.2022100060

Volume 44 Issue 16

Aug. 2023

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Science and Technology of Food Industry > 2023 > 44(16): 139-146. > DOI: 10.13386/j.issn1002-0306.2022100060

YE Hong, ZHOU Xuerui, WANG Su, et al. Effects of Yeast-lactic Acid Bacteria Co-fermentation on the Quality of Navel Orange Beverage[J]. Science and Technology of Food Industry, 2023, 44(16): 139−146. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100060.

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Effects of Yeast-lactic Acid Bacteria Co-fermentation on the Quality of Navel Orange Beverage

School of Food Bioengineering, Xihua University, Chengdu 610036, China

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Received Date: October 09, 2022
Available Online: June 14, 2023

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Abstract

To study the fermentation characteristics of Saccharomyces cerevisiae (SC-125) and Lactobacillus plantarum (B-5) in mixed bacterial fermentation, fresh Jintang navel orange juice was used as raw material and inoculated with the two bacteria separately for co-fermentation, to investigate the changes in the number of viable bacteria, pH, reducing sugar, alcohol, and The changes of basic physicochemical indexes such as viable bacteria, pH, reducing sugar, alcoholic content and total phenols were investigated, and the scavenging ability of DPPH and hydroxyl radicals of navel orange drinks were examined. The results showed that the yeast in the co-fermented navel orange beverage increased rapidly to 8.0 CFU·mL⁻¹ and the reduced sugar content decreased rapidly during 0~36 h of fermentation compared with the navel orange drink prepared by single-strain fermentation. The final alcohol content of co-fermentation was 5.33%, which was lower than that of brewer's yeast SC-125 alone. The total phenolic content, DPPH radicals and hydroxyl radical scavenging capacity of co-fermentation increased, reaching 355.5 mg·L⁻¹, 78.82%, and 77.08%, respectively. Co-fermentation volatile components were more abundant, with 51 aroma substances detected, including 9 unique aroma substances, namely: Isopropyl format, ethyl undecanoate, isobutyl acetate, methyl butyrate, tetrahydro linalool, 1-tetradecanol, 2-pentanol, undecylenic aldehyde, 2-nitropropane, giving navel orange drinks nutty, coconut and rose aromas. The principal component analysis of the electronic nose showed that the co-fermentation was more sensitive to three sensors, W5S, W1S and W2S, mainly nitrogen oxides, short chain alkanes and ethanol. This study would provide a theoretical basis for the development of navel orange beverages.
- navel orange drink,
- Saccharomyces cerevisiae SC-125,
- Lactobacillus plantarum B-5,
- co-fermentation

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References

[1]	武松伟, 梁珊珊, 谭启玲, 等. 柑橘营养特性与“以果定肥”[J]. 华中农业大学学报,2021,40(1):12−21. [WU S W, NIANG S S, TAN Q L, et al. Nutritional characteristics of citrus and "determining fertilizer by fruit"[J]. Journal of Huazhong Agricultural University,2021,40(1):12−21. WU S W, NIANG S S, TAN Q L, et al. Nutritional characteristics of citrus and "determining fertilizer by fruit" [J]. Journal of Huazhong Agricultural University, 2021, 40(1): 12-21.
[2]	牛静琪, 徐维盛, 赵佳, 等. 国产脐橙与进口脐橙的营养成分比较研究[C]//中国营养学会第十五届全国营养科学大会论文汇编, 2022: 891 NIU J Q, XU W S, ZHAO J, et al, Comparative study on nutritional components of domestic and imported navel oranges [C]. Collection of Papers of the 15th National Conference on Nutrition Science of the Chinese Society of Nutrition, 2022: 891.
[3]	李杰民, 吴翠琼, 何彩梅, 等. 干型富川脐橙果酒酿造技术研究[J]. 酿酒科技,2019(12):60−64. [LI J M, WU C Q, HE C M, et al. Research on brewing technology of dry Fuchuan navel orange fruit wine[J]. Brewing Technology,2019(12):60−64. LI J M, WU C Q, HE C M, et al. Research on brewing technology of dry Fuchuan navel orange fruit wine[J]. Brewing Technology, 2019(12): 60-64.
[4]	邢晓莹, 于迪, 乔羽, 等. 山楂果醋混合菌种发酵工艺及香气成分的HS-SPME/GC-MS分析[J]. 中国调味品,2021,46(01):146−152. [XING X Y, YU D, QIAO Y, et al. Fermentation process of hawthorn fruit vinegar by mixed strains and analysis of aroma components by HS-SPME/GC-MS[J]. Chinese Condiment,2021,46(01):146−152. XING X Y, YU D, QIAO Y, et al. Fermentation process of hawthorn fruit vinegar by mixed strains and analysis of aroma components by HS-SPME/GC-MS [J]. Chinese Condiment, 2021, 46 (01): 146-152.
[5]	牟志勇, 杨昳津, 王光强, 等. 酵母菌的益生功能及在食品中的应用[J]. 食品科学,2021,42(15):309−318. [M0U Z Y, YANG Y J, WANG G Q, et al. The probiotic function of yeast and its application in food[J]. Food Science,2021,42(15):309−318. doi: 10.7506/spkx1002-6630-20200609-123 M0U Z Y, YANG Y J, WANG G Q, et al. The probiotic function of yeast and its application in food[J]. Food Science, 2021, 42(15): 309-318. doi: 10.7506/spkx1002-6630-20200609-123
[6]	成晓苑. 乳酸菌在发酵食品中的应用研究[J]. 粮食科技与经济,2020,45(11):126−127. [CHENG X Y. Research on the application of lactic acid bacteria in fermented food[J]. Food Technology and Economics,2020,45(11):126−127. CHENG X Y. Research on the application of lactic acid bacteria in fermented food[J]. Food Technology and Economics, 2020, 45(11): 126-127.
[7]	EFRIWATI, SUWANTO A, RAHAYU G et al. Population dynamics of yeasts and I acticacid Bacteria (LAB) during tempeh production[J]. Hayati Journal of Biosciences,2013,20(2):57−64. doi: 10.4308/hjb.20.2.57
[8]	赵修报, 程立坤, 付强, 等. 乳酸菌和酵母菌混合发酵制备谷物发酵饮料的工艺研究[J]. 中国酿造,2021,40(2):198−202. [ZHAO X B, CHEN L K, FU Q, et al. Study on the technology of cereal fermented beverage by mixed fermentation of lactic acid bacteria and yeast[J]. China Brewing,2021,40(2):198−202. ZHAO X B, CHEN L K, FU Q, et al. Study on the technology of cereal fermented beverage by mixed fermentation of lactic acid bacteria and yeast[J]. China Brewing, 2021, 40(2): 198-202.
[9]	SANTOS C C, LIBECK B S, SCHWANR F. Co-culture fermentation of peanut-soy milk for the development of a novel functional beverage[J]. International Journal of Food Scienceand Technology,2014,186(3):32−41.
[10]	ALINEGALVAO T M, CINTIA L R, DIASD R, et al. Combination of probiotic yeast and lactic acid bacteria as starter culture to produce maize-based beverages[J]. Food Research Intereational,2018,111:187−197. doi: 10.1016/j.foodres.2018.04.065
[11]	SUN SY, GONG HS, ZHAO K, et al. Co-inoculation of yeast and lactic acid bacteria to improve cherry wines sensory quality[J]. International Journal of Food Scienceand Technology,2013,48(9):1783−1790. doi: 10.1111/ijfs.12151
[12]	陈毅坚, 张建前, 胡秋月, 等. 乳酸菌与酵母菌混菌发酵新型乳制品的研究[J]. 中国乳品工业,2021,49(6):27−32. [CHEN Y J, ZHANG J Q, HU Q Y, et al. Study on new dairy products fermented by mixed bacteria of lactic acid bacteria and yeast[J]. China Dairy Industry,2021,49(6):27−32. CHEN Y J, ZHANG J Q, HU Q Y, et al. Study on new dairy products fermented by mixed bacteria of lactic acid bacteria and yeast[J]. 2021, 49(6): 27-32.
[13]	马立娟, 王超, 杜丽平, 等. 植物乳杆菌和酿酒酵母发酵菠萝汁的性能比较及产物分析[J]. 中国酿造,2018,37(3):72−77. [MA L J, WANG C, DU L P, et al. Performance comparison and product analysis of pineapple juice fermented by Lactobacillus plantarum and Saccharomyces cerevisiae[J]. Chinese Brewing,2018,37(3):72−77. MA L J, WANG C, DU L P, et al. Performance comparison and product analysis of pineapple juice fermented by Lactobacillus plantarum and Saccharomyces cerevisiae[J]. Chinese Brewing, 2018, 37(3): 72-77.
[14]	金晓帆. 乳酸菌和酵母菌混合发酵芒果浆的研究[D]. 海口: 海南大学, 2019 JIN X F. Study on the mixed fermentation of mango pulp with lactic acid bacteria and yeast[D]. Haikou: Hainan University, 2019.
[15]	国家质量监督检验检疫总局, 国家标准化管理委员会. GB/T 15038-2006 葡萄酒果酒通用试验方法[S]. 北京: 中国标准出版社, 2006 General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China. GB/T 15038-2006 General test method for wine and fruit wine[S]. China Standard Press, 2006.
[16]	高文军, 李卫红, 王喜明, 等. 3, 5-二硝基水杨酸法测定蔓菁中还原糖和总糖含量[J]. 中国药业,2020,29(09):113−116. [GAO W J, LI W H, WANG X M, et al. Determination of reducing sugar and total sugar in Brassica chinensis by 3, 5-dinitrosalicylic acid method[J]. China Pharmaceutical,2020,29(09):113−116. GAO W J, LI W H, WANG X M, et al. Determination of reducing sugar and total sugar in Brassica chinensis by 3, 5-dinitrosalicylic acid method[J]. China Pharmaceutical, 2020, 29(09): 113-116.
[17]	吴澎, 贾朝爽, 李向阳, 等. 响应面分析优化福林酚法测定樱桃酒中总酚的含量[J]. 食品工业科技,2018,39(20):200−206,211. [WU P, JIA Z S, LI X G, et al. Determination of total phenols in cherry wine by response surface methodology optimization of Folin phenol method[J]. Food Industry Technology,2018,39(20):200−206,211. WU P, JIA Z S, LI X G, et al. Determination of total phenols in cherry wine by response surface methodology optimization of Folin phenol method[J]. Food Industry Technology, 2018, 39(20): 200-206, 211.
[18]	FULGENCIO A, RESENDE G, TEIXEIRA M, et al. Determination of alcohol content in beers of different styles based on portable near-infrared spectroscopy and multivariate calibration[J]. Food Analytical Methods,2021,15:307−316.
[19]	KAEWKOD T, BOVONSOMBUT S, TRAGOOLPIA Y. Efficacy of kombucha obtained from green, oolong, and black teas on inhibition of pathogenic bacteria, antioxidation, and toxicity on colorectal cancer cell line[J]. Microorganisms,2019,7(12):700. doi: 10.3390/microorganisms7120700
[20]	HUI Y, JIN-LI H, CHUANG W. Anti-oxidation and anti-aging activity of polysaccharide from Malus micromoles Makino fruit wine[J]. International Journal of Biologicals Macromolecules,2019,121:1203−1212. doi: 10.1016/j.ijbiomac.2018.10.096
[21]	蔡婷, 宋菲菲, 秦佳, 等. 天然野樱桃果酒的酿造及香气成分分析[J]. 中国酿造,2015,34(06):145−149. [CAI T, SONG FF, QIN J, et al. Brewing and aroma component analysis of natural wild cherry wine[J]. Chinese Brewing,2015,34(06):145−149. CAI T, SONG FF, QIN J, et al. Brewing and aroma component analysis of natural wild cherry wine[J]. Chinese Brewing, 2015, 34(06): 145-149.
[22]	DESOUZA M P, BATAGLION, G A, DEALMEIDA, R A, et al. Phenolic and aroma compositions of pitomba fruit (Talisa esculenta Radek.) assessed by LC-MS/MS and HS-SPME/GC-MS[J]. Food Research International,2016,83:87−94. doi: 10.1016/j.foodres.2016.01.031
[23]	TAN J, XU J. Applications of electronic nose (e-nose) and electronic tongue (e-tongue) in food quality-related properties determination: A review[J]. Artificial Intelligence in Agriculture,2020,4:104−115. doi: 10.1016/j.aiia.2020.06.003
[24]	缪璐, 何善廉, 莫佳琳, 等. 电子鼻技术在朗姆酒分类识别中的应用研究[J]. 广西糖业,2016(4):24−33. [MIAO L, HE S L, MO J L, et al. Research on the application of electronic nose technology in rum classification and recognition[J]. Guangxi Sugar Industry,2016(4):24−33. doi: 10.3969/j.issn.1007-4732.2016.04.005 MIAO L, HE S L, MO J L, et al. Research on the application of electronic nose technology in rum classification and recognition[J]. Guangxi Sugar Industry, 2016(4): 24-33. doi: 10.3969/j.issn.1007-4732.2016.04.005
[25]	SWIEGERS J H, BARTOWSKY E J, HENSCHKE P, et al. Yeast and bacterial modulation of wine aroma and flavour[J]. Australian Journal of Grape and Wine Research,2005,11(2):139−173. doi: 10.1111/j.1755-0238.2005.tb00285.x
[26]	WANG Z, XU K, CAI R, et al. Construction of recombinant Fusan yeasts for the production of cider with low alcohol and enhanced aroma[J]. European Food Research and Technology,2020,246:745−757. doi: 10.1007/s00217-020-03436-9
[27]	叶萌祺. 苹果酒酿造过程香气物质调控及FT-NIRS分析方法研究[D]. 咸阳: 西北农林科技大学, 2015 YE M Q. Control of aroma substances in cider brewing process and FT-NIRS analysis method[D]. Xianyang: Northwest Agriculture and Forestry University, 2015.
[28]	王东伟, 黄燕芬, 肖默艳, 等. 猕猴桃果酒发酵条件优化及其抗氧化特性研究[J]. 食品安全质量检测学报,2019,10(6):1619−1625. [WANG D W, HUANG Y F, XIAO M, et al. Optimization of fermentation conditions and antioxidant properties of kiwifruit wine[J]. Journal of Food Safety and Quality Inspection,2019,10(6):1619−1625. doi: 10.3969/j.issn.2095-0381.2019.06.034 WANG D W, HUANG Y F, XIAO M, et al. Optimization of fermentation conditions and antioxidant properties of kiwifruit wine[J]. Journal of Food Safety and Quality Inspection, 2019, 10(6): 1619-1625. doi: 10.3969/j.issn.2095-0381.2019.06.034
[29]	马博文. 巴斯德毕赤酵母和酿酒酵母混菌发酵黄桃酒工艺优化及风味的研究[D]. 上海: 上海应用技术大学, 2020 MA B W. Study on technology optimization and flavor of yellow peach wine by mixed fermentation of Pichia pastoris and Saccharomyces cerevisiae[D]. Shanghai: Shanghai University of Applied Technology, 2020.
[30]	赵一凡. 梨酒的酿造工艺研究[D]. 石家庄: 河北科技大学, 2019 ZHAO Y F. Research on brewing technology of pear wine[D]. Shijiazhuang: Hebei University of Science and Technology, 2019.
[31]	ZHANG S R, XING X, CHU Q, et al. Impact of co-culture of Lactobacillus plantarum and Oenococcus oeniat different ratios on malolactic fermentation, volatile and sensory characteristics of mulberry wine[J]. LWT,2022,169:113995. doi: 10.1016/j.lwt.2022.113995
[32]	张雁凌, 张雁南, 刘刚. 乳酸菌和酵母菌的添加对低盐酱油品质的影响[J]. 中国调味品,2020,45(12):45−47,51. [ZHANG Y L, ZHANG Y N, LIU G. Effect of lactic acid bacteria and yeast on the quality of low-salt soy sauce[J]. Chinese Condiment,2020,45(12):45−47,51. doi: 10.3969/j.issn.1000-9973.2020.12.010 ZHANG Y L, ZHANG Y N, LIU G. Effect of lactic acid bacteria and yeast on the quality of low-salt soy sauce[J]. Chinese Condiment, 2020, 45(12): 45-47, 51. doi: 10.3969/j.issn.1000-9973.2020.12.010
[33]	孙佳勰, 康文怀, 李慧, 等. 桑葚果酒发酵过程中的香气成分变化[J]. 现代食品科技,2020,36(8):307−316,201. [SUN J X, KANG W H, LI H, et al. Changes of aroma components in mulberry wine during fermentation[J]. Modern Food Technology,2020,36(8):307−316,201. SUN J X, KANG W H, LI H, et al. Changes of aroma components in mulberry wine during fermentation[J]. modern food technology, 2020, 36(8): 307-316, 201.
[34]	蔡健, 施佳康. 黄桃酸奶的加工工艺研究[J]. 食品科技,2011(2):76−79. [CAI J, SHI J K. Study on processing technology of yellow peach yoghurt[J]. Food Science and Technology,2011(2):76−79. CAI J, SHI J K. Study on processing technology of yellow peach yoghurt. Food Science and Technology, 2011(2): 76-79.
[35]	谭琰俐, 吴泳, 刘品, 等. 基于HS-SPME-GC-MS的不同醋龄河溪香醋香气成分比较[J]. 食品与机械,2022,38(8):80−86. [TAN Y L, WU Y, LIU P, et al. Comparison of aroma components of hexi vinegar of different vinegar ages based on HS-SPME-GC-MS[J]. Food and Machinery,2022,38(8):80−86. TAN Y L, WU Y, LIU P, et al. Comparison of aroma components of hexi vinegar of different vinegar ages based on HS-SPME-GC-MS [J]. Food and Machinery, 2022, 38(8): 80-86.
[36]	ZHU D S, REN X J, WEI L W, et al. Collaborative analysis on difference of apple fruits flavour using electronic nose and electronic tongue[J]. Scientia Horticulture,2019,26:178−183.

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