Anthocyanin Distribution and Sampling Method in Colored Potato Tubers

CUI Lingyan; YANG Yan; WANG Qiong; XU Xiaoyu; LI Kaifeng; LI Maoxing; BAI Lei; GUO Huachun

doi:10.13386/j.issn1002-0306.2021050128

Volume 43 Issue 4

Feb. 2022

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Science and Technology of Food Industry > 2022 > 43(4): 293-299. > DOI: 10.13386/j.issn1002-0306.2021050128

CUI Lingyan, YANG Yan, WANG Qiong, et al. Anthocyanin Distribution and Sampling Method in Colored Potato Tubers[J]. Science and Technology of Food Industry, 2022, 43(4): 293−299. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021050128.

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Anthocyanin Distribution and Sampling Method in Colored Potato Tubers

1.
College of Agronomy and Biotechnology, Yunnan Agriculture University, Kunming 650201, China
2.
Faculty of Mechanical and Electrical Engineering, Yunnan Agriculture University, Kunming 650201, China

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Received Date: May 16, 2021
Available Online: December 16, 2021

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Abstract

Abstract

In order to investigate the difference of anthocyanin distribution in potato tuber and explore the most applicable method for sampling economically and scientifically, three colored potato materials, ‘Heijingang (variety)’, ‘21-1 (line)’ and ‘Jianchuanhong (variety)’ with various pigment accumulation were selected for the analysis of the tuber anthocyanin content depend on the size (large, middle and small) and length (longitudinal and the lateral) of the tuber, as well as the angle (transversely, vertically and diagonally) from where the tuber was cut for sampling. The results showed that the distribution of the potato anthocyanins in the same species was significantly different due to the material and size differences (P<0.05). In the same tuber, the allocation of anthocyanins was of significant difference along the horizontal area, which was significantly higher in the potato flesh (P<0.05). Specifically, the anthocyanins level in the skin of ‘Jianchuanhong’ was about 7-fold higher than the flesh, while it was 2.5-fold and 1.5-fold higher in ‘Heijingang’ and ‘21-1’ respectively. By comparison, in the longitudinal distribution, anthocyanins contents in the top and umbilical regions of the ‘Heijingang’ and ‘21-1’ were both significantly higher than the middle part, whereas the top of the ‘Jianchuanhong’ was significantly higher than the middle and the umbilical area (P<0.05). Relatively, the three methods utilized to cut the tubers from three angles did not reflect significant differences in terms of the anthocyanin quantification (P˃0.05), showing that sample lesser amount of the tuber through the diagonal cut might be a much more efficient and economical way for the potato pigment analysis. Lastly, this study also compared the analytical effects between tuber grinding and pulping, demonstrating that the pulping method could cause the anthocyanin loss drastically (P<0.05), which was not an appropriate way for the potato tuber pigment quantification. Thus, cutting the potato tuber from a morphologically diagonal angle with lesser amount would be a quite favorable approach for the lab detection of potato tuber anthocyanins.
- colored potato,
- anthocyanin,
- distribution difference,
- sampling methods

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References (36)

References

[1]	庄维兵, 刘天宇, 束晓春, 等. 植物体内花青素苷生物合成及呈色的分子调控机制[J]. 植物生理学报,2018,54(11):1630−1644. [ZHUANG W B, LIU T Y, SHU X C, et al. The molecular regulation mechanism of anthocyanin biosynthesis and coloration in plants[J]. Plant Physiology Journal,2018,54(11):1630−1644.
[2]	RAIGOND P, SINGH B, DUTT S, et al. Potato nutrition and food security[M]. Singapore: Springer, 2020: 173-189.
[3]	李仲叶, 韩豪, 江海, 等. 花色苷抗氧化活性的研究现状及展望[J]. 食品工业,2019,40(8):245−250. [LI Z Y, HAN H, JIANG H, et al. Research status and prospects of the antioxidant activity of anthocyanins[J]. The Food Industry,2019,40(8):245−250.
[4]	BRUNETTI C, M D FERDINANDO, FINI A, et al. Flavonoids as antioxidants and developmental regulators: Relative significance in plants and humans[J]. International Journal of Molecular Sciences,2013,14(2):3540−3555. doi: 10.3390/ijms14023540
[5]	位路路. 黑果腺肋花楸花色苷提取物对脂多糖诱导巨噬细胞炎症的抑制作用[D]. 沈阳: 沈阳农业大学, 2018. WEI L L. The inhibition of aronia melanocarpa anthocyanin extracts on macrophages inflammation induced by lipopolysaccharide[D]. Shenyang: Shenyang Agricultural University, 2018.
[6]	饶炎炎, 桑英, 唐琳琳, 等. 红树莓果酒发酵过程中功效成分、香气物质及体外降血糖功效的动态变化[J]. 食品科学,2020,41(6):222−230. [RAO Y Y, SANG Y, TANG L L, et al. Dynamic changes in aroma, functional components and hypoglycemic effect in vitro of red raspberry wine during fermentation[J]. Food Science,2020,41(6):222−230. doi: 10.7506/spkx1002-6630-20190408-062
[7]	LIPPERT E, RUEMMELE P, OBERMEIER F, et al. Anthocyanins prevent colorectal cancer development in a mouse model[J]. Digestion,2017,95(4):275−280. doi: 10.1159/000475524
[8]	CHAREPALLI V, REDDIVARI L, RADHAKRISHNAN S, et al. Anthocyanin-containing purple-fleshed potatoes suppress colon tumorigenesis via elimination of colon cancer stem cells[J]. Journal of Nutritional Biochemistry,2015:1641−1649.
[9]	黄旭东. 紫色马铃薯不同部位花色苷抗前列腺癌细胞活性研究[D]. 杭州: 浙江大学, 2017. HUANG X D. Studies on the anti-prostate cancer cell viability of anthocyanins from different parts of purple potato[D]. Hangzhou: Zhejiang University, 2017.
[10]	尤诗莹. 功能性食品添加剂领域——天然食用色素的研究进展[J]. 饮食科学,2017,12(380):36−37. [YOU S Y. Research progress of natural food coloring in the field of functional food additives[J]. Diet Science,2017,12(380):36−37.
[11]	MARKAKIS P. Anthocyanins as food additives[J]. Anthocyanins as Food Colors,1982:245−253.
[12]	WALLACE, GIUSTI. Anthocyanins—nature’s bold, beautiful, and health-promoting colors[J]. Foods,2019,8(11):550. doi: 10.3390/foods8110550
[13]	SINGH J, MEEHNIAN H, GUPTA P, et al. Biotechnological Approaches: Food colours[M]. Springer India, 2020.
[14]	AKHTAR, M HUMAYOUN, ZAHEER, et al. Potato production, usage, and nutrition-a review[J]. Critical Reviews in Food Science & Nutrition, 2016, 56(5): 711-721 .
[15]	MISHRA T, RAIGOND P, THAKUR N, et al. Recent updates on healthy phytoconstituents in potato: A nutritional depository[J]. Potato Research,2020(2):1−21.
[16]	宋威武, 吴承金, 陈火云, 等. 彩色马铃薯花色苷及育种研究进展[C]//马铃薯产业与健康消费(2019). 2019: 133−137. SONG W W, WU C G, CHEN H Y, et al. Research progress on anthocyanins and breeding of colored potato[C]// Potato industry and health consumption(2019). 2019: 133−137.
[17]	殷丽琴, 彭云强, 钟成, 等. 高效液相色谱法测定8个彩色马铃薯品种中花青素种类和含量[J]. 食品科学,2015(18):143−147. [YIN L Q, PENG Y Q, ZHONG C, et al. Determination of anthocyanidin composition of different pigmented potato (Solanum tuberosum L.) cultivars by HPLC[J]. Food Science,2015(18):143−147. doi: 10.7506/spkx1002-6630-201518026
[18]	张静, 郭华春, 姚春雪. 11个云南地方马铃薯品种块茎的花色苷含量及其分布[C]//马铃薯产业与粮食安全(2009). 2009. ZHANG J, GUO H C, YAO C X. Anthocyanins content and distribution in tubers of 11 Yunnan native potato varieties[C]// Potato industry and food security(2009). 2009.
[19]	JANSEN G, FLAMMEL W. Coloured potatoes (Solanum tuberosum L.) – anthocyanin content and tuber quality[J]. Genetic Resources & Crop Evolution,2006,53(7):1321.
[20]	白粉娥, 成宇峰. 两种彩色马铃薯品种花色苷成分分析及其总抗氧化活性的比较研究[J]. 保鲜与加工,2018,18(2):108−113. [BAI F E, CHENG Y F. Comparative study of anthocyanins component and total antioxidant activity between two colorful potato cultivars[J]. Storage and Process,2018,18(2):108−113. doi: 10.3969/j.issn.1009-6221.2018.02.018
[21]	LEWIS C E, WALKER J R L, LANCASTER J E, et al. Light regulation of anthocyanin, flavonoid and phenolic acid biosynthesis in potato minitubers in vitro[J]. Australian Journal of Plant Physiology,1998,25(8):915−922.
[22]	LEE J, RENNAKER C, WROLSTAD R E. Correlation of two anthocyanin quantification methods: HPLC and spectrophotometric methods[J]. Food Chemistry,2008,110(3):782−786. doi: 10.1016/j.foodchem.2008.03.010
[23]	殷丽琴, 韦献雅, 钟成, 等. 不同品种彩色马铃薯总花色苷含量与总抗氧化活性[J]. 食品科学,2014,35(5):96−100. [YIN L Q, WEI X Y, ZHONG C, et al. Total anthocyanin content and total antioxidant activities in different varieties of pigmented potato (Solanum tuberosum L.)[J]. Food Science,2014,35(5):96−100. doi: 10.7506/spkx1002-6630-201405019
[24]	FULEKI T, FRANCIS F J. Quantitative methods for anthocyanins. 1. Extraction and determination of total anthocyanin in cranberries[J]. Journal of Foodence,1968,33(1):72−77.
[25]	FULEKI T, FRANCIS F J. Quantitative methods for anthocyanins. : 2. Determination of total anthocyanin and degradation index for cranberry juice[J]. Journal of Foodence,1968,33(1):78−83.
[26]	LEE J, DURST, ROBERT W, et al. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study[J]. Journal of AOAC International,2005,88(5):69−78.
[27]	WROLSTAD R E. Aoac official method 2005.02: Total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method[M]. 2005: 1−2.
[28]	李倩, 柳俊, 谢从华, 等. 彩色马铃薯块茎形成和贮藏过程中花色苷变化及抗氧化活性分析[J]. 园艺学报,2013,40(7):1309−1317. [LI Q, LIU J, XIE C H, et al. Variation of anthocyanin of colored-flesh potato tubers and measurement of their antioxidant activity during the tuber development and storage[J]. ACTA Horticulturae Sinica,2013,40(7):1309−1317.
[29]	YL A, JL B, XZ B, et al. Genome-wide analysis of MYB gene family in potato provides insights into tissue-specific regulation of anthocyanin biosynthesis[J]. Horticultural Plant Journal,2020,7(2):129−141.
[30]	OERTEL A, MATROS A, HARTMANN A, et al. Metabolite profiling of red and blue potatoes revealed cultivar and tissue specific patterns for anthocyanins and other polyphenols[J]. Planta,2017,246(2):281−297. doi: 10.1007/s00425-017-2718-4
[31]	SHEPHERD T, DOBSON G, VERRALL S R, et al. Potato metabolomics by GC-MS: What are the limiting factors?[J]. Metabolomics,2007,3(4):475−488. doi: 10.1007/s11306-007-0058-2
[32]	GRIFFITHS D W, DALE M. Effect of light exposure on the glycoalkaloid content of solanum phureja tubers[J]. Journal of Agricultural & Food Chemistry,2001,49(11):5223−5227.
[33]	范志红. 怎样减少鲜榨汁的营养损失?[J]. 健康之家,2014(11):85. [FAN Z H. How to reduce the nutritional losses of fresh juicing?[J]. Health,2014(11):85.
[34]	范祺祺, 董文, 熊兴耀, 等. 植物地下器官花色苷合成及其调控研究进展[J]. 中国蔬菜,2019(6):21−28. [FAN Q Q, DONG W, XIONG X Y, et al. Advances in anthocyanin synthesis and regulation in plant subterranean organs[J]. China Vegetables,2019(6):21−28.
[35]	YING L, YURY T, SCHOUTEN R E, et al. Anthocyanin biosynthesis and degradation mechanisms in solanaceous vegetables: A review[J]. Frontiers in Chemistry,2018,52(6):1−17.
[36]	JAAKOLA, LAURAL. New insights into the regulation of anthocyanin biosynthesis in fruits[J]. Trends in Plant Science,2013,18(9):477−483. doi: 10.1016/j.tplants.2013.06.003

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