Protective Effect of <i>Acanthopanax trifoliatus </i>Extract on Rats with Alcoholic Liver Injury

LI Dongmei; LI Zhiyang; YANG Jun; HAO Zhiming; YANG Jie; LIN Chunhua

doi:10.13386/j.issn1002-0306.2021010238

Volume 42 Issue 18

Sep. 2021

Turn off MathJax

Article Contents

Abstract

References

Science and Technology of Food Industry > 2021 > 42(18): 380-386. > DOI: 10.13386/j.issn1002-0306.2021010238

LI Dongmei, LI Zhiyang, YANG Jun, et al. Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury[J]. Science and Technology of Food Industry, 2021, 42(18): 380−386. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021010238.

Citation:

PDF (950 KB)

Protective Effect of Acanthopanax trifoliatus Extract on Rats with Alcoholic Liver Injury

Institute of Tropical Agriculture and Forestry, Guangdong AIB Polytechnic College, Guangzhou 510503, China

More Information

Received Date: January 31, 2021
Available Online: July 16, 2021

Graphical Abstract

Abstract

Abstract

Objective: To explore the hepatoprotective effects of different extracts of Acanthopanax trifoliatu, and provide a basis for the research and development of Acanthopanax trifoliatus anti-alcohol and liver-protecting functional foods. Method: Rats were fed by liquor (56% ethanol) to induce acute liver injury models, to study the effects of alcohol extract and water extract of Acanthopanax trifoliatus on the alcohol tolerance, sleep and sober time of rats. The concentration of ethanol in blood and the activites of ALT & AST in serum, activites of ADH, ALDH, SOD and contents of GSH and MDA in rat liver tissue were measured, and the changes of liver cell morphology by slice microscopic examination was observed. Results: Compared with the alcohol model group, except for the low-dose water extract group, there was no significant change in the drunken time (P>0.05), the various doses extract significantly prolonged the alcohol tolerance time and shortened the sleep and sober time (P<0.05, P<0.01); the various doses extract significantly reduced the ethanol mass concentration (P<0.01); except for the low-dose water extract group, there was no significant change in AST activity (P>0.05), all dose groups of extract could significantly inhibit the increase in rat serum ALT and AST activities caused by alcohol (P<0.05); all extracts significantly increased the activity of ADH, ALDH, SOD and GSH content in liver tissues (P<0.01), and reduced the content of MDA in liver tissues (P<0.01). The results of hepatocyte microscopy showed that all extracts could reduce the degree of liver cell degeneration caused by alcohol to a certain extent. Conclusion: Both the alcoholic and water extracts had good anti-alcoholic and liver-protective effects, in comparison, the alcohol extract was slightly better than the water extract.
- Acanthopanax trifoliatus,
- alcohol extract,
- water extract,
- alcoholic liver injury,
- rat,
- anti-alcohol and liver-protecting

FullText(HTML)

References (30)

References

[1]	张焜, 程书朋, 武文敬, 等. 簕菜化学成分、生物活性及其安全性研究进展[J]. 海峡药学,2017,29(4):1−5. [Zhang K, Cheng S P, Wu W J, et al. Research advance in chemical compositions, bioactivities and safety of Acanthopanax trifoliatus[J]. Strait Pharmaceutical Journal,2017,29(4):1−5. doi: 10.3969/j.issn.1006-3765.2017.04.001
[2]	修程蕾, 胡心怡, 屠宇帆, 等. 五加属植物白簕活性成分及其应用研究进展[J]. 亚热带植物科学,2016,45(1):90−94. [Xiu C L, Hu X Y, Tu Y F, et al. Advance in active ingredients of Eleutherococcus trifoliatus and its functional development[J]. Subtropical Plant Science,2016,45(1):90−94. doi: 10.3969/j.issn.1009-7791.2016.01.020
[3]	林春华, 林伟君, 谭雪, 等. 野生蔬菜簕菜品质及急性毒性试验研究[J]. 现代食品科技,2009,25(2):201−202, 171. [Lin C H, Lin W J, Tan X, et al. Quality and acute toxication test of a wild vegetable Acanthopanax trifoliatus[J]. Modern Food Science and Technology,2009,25(2):201−202, 171.
[4]	林春华, 乔燕春, 谭雪, 等. 野生蔬菜白簕的安全性评价[J]. 广东农业科学,2014,41(8):47−51. [Lin C H, Qiao Y C, Tan X, et al. Safety evaluation of Acanthopanax trifoliatus, a wild vegetable[J]. Guangdong Agricultural Sciences,2014,41(8):47−51. doi: 10.3969/j.issn.1004-874X.2014.08.012
[5]	Sithisarn P, Jarikasem S, Thisayakorn K. Acanthopanax trifoliatus, a potential adaptogenic thai vegetable for health supplement[M]. Bangkok: InTech, 2011: 253-268.
[6]	Hamid R A, Kee T H, Othman F. Anti-inflammatory and anti-hyperalgesic actives of Acanthopanax trifoliatus (L.)Merr. leaves[J]. Pharmacognosy Research,2013,5(2):129−133. doi: 10.4103/0974-8490.110544
[7]	Tzu M C, Po C H, Shyh H S H, et al. Acanthopanax trifoliatus inhibits lipopolysaccharide-induced inflammatory respond in vitro and in vivo[J]. Medical Science,2015,31(10):499−509.
[8]	劳景辉, 潘超美, 喻勤, 等. 白簕提取物抑制痤疮丙酸杆菌及美白活性研究[J]. 中国现代中药,2016,18(9):1120−1124. [Lao J H, Pan C M, Yu Q, et al. Study on antimicrobial activity against Propionibacterium acnes and whitening effect of extract of Eleutherococcus trifoliatus in vitro[J]. Modern Chinese Medicine,2016,18(9):1120−1124.
[9]	郑诗嘉. 白簕多糖的组成分析及其降血糖作用机制研究[D]. 广州: 广东药科大学, 2019. Zheng S J. Composition analysis and hypoglycemic mechanism of polysaccharides from Acanthopanax trifoliatus[D]. Guangzhou: Guangdong Pharmaceutical University, 2019.
[10]	张旭红. 白簕茎多糖的分离纯化及其降糖活性研究[D]. 广州: 广东药科大学, 2016. Zhang X H. Study on the isolation, purification and hypoglycemic activities of polysaccharides from the stem of Acanthopanax trifoliatus[D]. Guangzhou: Guangdong Pharmaceutical University, 2016.
[11]	Li D, Du Z, Li C, et al. Potent inhibitory effect of terpenoids from Acanthopanax trifoliatus on growth of PC-3 prostate cancer cells in vitro and in vivo is associated with suppression of NF-κB and STAT3 signalling[J]. Journal of Functional Foods,2015,15:274−283. doi: 10.1016/j.jff.2015.03.035
[12]	Nguyen T T, Le T H N, Dao D, et al. Synthesis and cytotoxic activity of derivatives of 24-nor-lupane-triterpenoid acids isolated from Acanthopanax trifoliatus[J]. Chemistry of Natural Compounds,2015,51(2):276−282. doi: 10.1007/s10600-015-1261-4
[13]	张元. 簕菜有效成分提取分离及其抗氧化活性研究[D]. 广州: 广东工业大学, 2015. Zhang Y. Extraction and separation of effective components and study on antioxidant activity of Acanthopanax trifoliatus[D]. Guangzhou: Guangdong University of Technology, 2015.
[14]	王宝庆, 郭鑫磊, 刘莹, 等. 白簕化学成分及其药理活性研究进展[J]. 北方园艺,2018(13):162−168. [Wang B Q, Guo X L, Liu Y, et al. Research advances in chemical constituents and pharmacological activities of Acanthopanax trifoliatus[J]. Northern Horticulture,2018(13):162−168.
[15]	高林晓, 郭蒙, 杨再波, 等. 五加科植物刺三加研究新进展[J]. 中华中医药杂志,2018,33(4):1466−1469. [Gao L X, Guo M, Yang Z B, et al. New research progress of Acanthopanan trifoliatus of Araliaceae[J]. Chinese Journal of Traditional Chinese Medicine,2018,33(4):1466−1469.
[16]	Torruellas C, French S W, Medici V. Diagnosis of alcoholic liver disease[J]. World Journal of Gastroenterology,2014,20(33):11684−11699. doi: 10.3748/wjg.v20.i33.11684
[17]	Sahlman P, Nissinen M, Pukkala E, et al. Incidence, survival and cause-specific mortality in alcoholic liver disease: A population-based cohort study[J]. Scandinavian Journal of Gastroenterology,2016,51(8):961−966. doi: 10.3109/00365521.2016.1157889
[18]	Bang H A, Kwon Y H, Lee M J, et al. Trends in the epidemiological aspects and mortality of alcoholic liver disease in Korea in the decade between 2000 and 2009[J]. Journal of Clinical Medicine Research,2015,7(2):91−96. doi: 10.14740/jocmr1975e
[19]	张译文, 李昱锦, 胡冰芳, 等. 三种大鼠酒精性肝病短期模型的评价[J]. 药学学报,2018,53(2):236−243. [Zhang Y W, Li Y J, Hu B F, et al. Evaluation on three short-term animal models of alcoholic liver disease[J]. Acta Pharmaceutica Sinica,2018,53(2):236−243.
[20]	罗安玲. 葛根、藤茶、玉米低聚肽复合组方对大鼠的解酒保肝作用研究[D]. 上海: 上海交通大学, 2019. Luo A L. Study on the anti-alcohol and hepatoprotective effect of compound of Pueraria, Ampelopsis grossedentata, and corn oligopeptides against alcoholic liver injury in mice[D]. Shanghai: Shanghai Jiaotong University, 2019.
[21]	蔡凌云, 肖娟, 韩素菊, 等. 白簕叶中黄酮成分的鉴定和含量测定[J]. 绵阳师范学院学报,2010,29(11):78−80, 84. [Cai L X, Xiao J, Han S J, et al. Component evaluation and content determination in the leaves of Acanthopanan trifoliatus[J]. Journal of Mianyang Normal University,2010,29(11):78−80, 84. doi: 10.3969/j.issn.1672-612X.2010.11.017
[22]	聂永心. 黄伞子实体多糖的分离纯化、结构鉴定及生物活性的研究[D]. 泰安: 山东农业大学, 2011. Nie Y X. Isolation, purification, structure identification and biological activity of polysaccharides from yellow umbrella fruit body[D]. Taian: Shandong Agricultural University, 2011.
[23]	王翰华, 杨晓春. 野葛叶水提物、醇提物对急性酒精中毒大鼠的解酒护肝作用[J]. 中成药,2018,40(12):2744−2747. [Wang H H, Yang X C. The effect of water extract and alcohol extract of Pueraria lobata leaf on antialcoholism and liver protection in mice with acute alcoholism[J]. Chinese Traditional Patent Medicine,2018,40(12):2744−2747. doi: 10.3969/j.issn.1001-1528.2018.12.028
[24]	杨建, 周先叶. 印度獐芽菜提取物解酒保肝功效的研究[J]. 今日药学,2017,27(7):447−450. [Yang J, Zhou Y X. Study on the anti-alcoholism and protecting liver effects of Swertia chirata extracts[J]. Pharmacy Today,2017,27(7):447−450. doi: 10.12048/j.issn.1674-229X.2017.07.005
[25]	李竹英, 唐承爱. 麦冬多糖解酒保肝作用的实验研究[J]. 湘南学院学报(医学版),2017,19(4):20−24. [Li Z Y, Tang C A. Experimental study on effects of Ophiopogonis japonicas polysaccharides on the alleviation of alcoholism and protection of liver[J]. Journal of Xiangnan University(Medical Sciences),2017,19(4):20−24.
[26]	陈丰. 枳葛解酒保肝方对大鼠酒精性肝损伤的防治作用及机制探讨[D]. 北京: 北京中医药大学, 2018. Chen F. Prevention and treatment and its mechanism of Zhige Jiejiu Baogan Fang on alcoholic liver injury in rats[D]. Beijing: Beijing University of Chinese Medicine, 2018.
[27]	杨毅雯. 紫茉莉对酒精性肝损伤大鼠ALT、AST、T-SOD影响的研究[D]. 大理: 大理学院, 2013. Yang Y W. The effect of Mirabilis on ALT, AST and T-SOD in rats with alcoholic liver injury[D]. Dali: Dali University, 2013.
[28]	Ohgo H, Yokoyama H, Hirose H, et al. Significance of ALT/AST ratio for specifying subjects with metabolic syndrome in its silent stage[J]. Diabetes & Metabolic Syndrome Clinical Research & Reviews,2009,3(1):3−6.
[29]	B Gao, R Bataller. Alcoholic liver disease: Pathogenesis and new therapeutic targets[J]. Gastroenterology,2011,141(5):1572−1585. doi: 10.1053/j.gastro.2011.09.002
[30]	田姮. 蓝莓解酒护肝作用及其机理的研究[D]. 沈阳: 沈阳农业大学, 2017. Tian H. The hepatoprotective effect of blueberry against the alcoholic liver injury in rats and its mechanisms[D]. Shenyang: Shenyang Agricultural University, 2017.

[1]	ZHOU Xiaoyue, YAO Lina, TAO Wenchu, BEN Dongxu, ZHAO Fangfang, XUE Yuqing, LI Yanjun, SHU Zhicheng. Effects of Microcrystalline Cellulose Concentration in Complex Colloidal System on Stability of Chestnut Suspension[J]. Science and Technology of Food Industry, 2023, 44(6): 121-127. DOI: 10.13386/j.issn1002-0306.2022080044
[2]	HU Wanting, LUO Man, LU Jieyi, HUANG Yuchun, QIAO Dongling. Preparation and Properties of Agar/Konjac Glucomannan/Ethyl Cellulose Composite Film[J]. Science and Technology of Food Industry, 2022, 43(11): 260-266. DOI: 10.13386/j.issn1002-0306.2021090150
[3]	SONG Zuohui, HOU Hanxue, WANG Wentao, ZHANG Jinli. Preparation, Characterization and in Vitro Release of Agar/Maltodextrin Sustained-release Capsules[J]. Science and Technology of Food Industry, 2021, 42(22): 178-184. DOI: 10.13386/j.issn1002-0306.2021020240
[4]	ZHANG Chenghao, JIANG Zedong, LI Hebin, NI Hui, ZHU Yanbing, LI Qingbiao. Preparation and Characterization of Mutant Arylsulfatase-Modified Agar[J]. Science and Technology of Food Industry, 2021, 42(5): 39-44. DOI: 10.13386/j.issn1002-0306.2019100059
[5]	WANG Zhiguo, ZHONG Chunyan, ZHANG Weimin. Control of Spontaneous Mutation of Komagataeibacter xylinus by Agar[J]. Science and Technology of Food Industry, 2021, 42(4): 103-107,201. DOI: 10.13386/j.issn1002-0306.2020050275
[6]	ZHU Yun-heng, JIANG Ze-dong, NI Hui, ZHU Yan-bing, XIAO An-feng, LI Qing-biao. Study on the Peparation of High Tansparent Agar[J]. Science and Technology of Food Industry, 2019, 40(21): 149-153,158. DOI: 10.13386/j.issn1002-0306.2019.21.024
[7]	AN Ding, ZHANG Qiu-jun, NI Hui, JIANG Ze-dong, XIAO An-feng. Study on Compound of Agar to Exploit Yoghourt Stabilizer[J]. Science and Technology of Food Industry, 2018, 39(15): 219-222,233. DOI: 10.13386/j.issn1002-0306.2018.15.039
[8]	XIE Jian-hua, XIE Bing-qing, ZHANG Li-hong, WANG Wen-cheng, PANG Jie, ZHANG Min. Study on shear rheological properties of Konjac glucomannan and Agar blends[J]. Science and Technology of Food Industry, 2017, (16): 1-4. DOI: 10.13386/j.issn1002-0306.2017.16.001
[9]	LIU Shi-lin, ZHU Feng, LIN Sheng-nan, HUANG Jin-cheng, LI Tian-jiao, WANG Hong-li, LIN Xiang-yang. Research of strength and relaxation properties of agar gel[J]. Science and Technology of Food Industry, 2017, (13): 85-89. DOI: 10.13386/j.issn1002-0306.2017.13.016
[10]	TIAN Fen, OU Kai, GAO Xing-hua, WANG Yin-juan, LI Xiao-hong, WU Wei-du. The rheological properties of agar and their effects on stability of drinking yoghurt[J]. Science and Technology of Food Industry, 2016, (06): 160-163. DOI: 10.13386/j.issn1002-0306.2016.06.024