Purification and Antioxidant Properties of Anthocyanins from Jingle <i>Lycium ruthenicum</i>

Hongxia YUAN; Qianyi HOU; Shan DU; Huan TIAN; Qingshan LI

doi:10.13386/j.issn1002-0306.2020070221

Volume 42 Issue 9

Apr. 2021

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Science and Technology of Food Industry > 2021 > 42(9): 173-178. > DOI: 10.13386/j.issn1002-0306.2020070221

YUAN Hongxia, HOU Qianyi, DU Shan, et al. Purification and Antioxidant Properties of Anthocyanins from Jingle Lycium ruthenicum [J]. Science and Technology of Food Industry, 2021, 42(9): 173−178. (in Chinese with English abstract). doi: 10.13386/ j.issn1002-0306.2020070221.

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Purification and Antioxidant Properties of Anthocyanins from Jingle Lycium ruthenicum

Hongxia YUAN^{1, 2,},
Qianyi HOU^{1, 2},
Shan DU¹,
Huan TIAN¹,
Qingshan LI^{1, 2, ,}

1.
School of Chinese Medicine and Food Engineering, Shanxi University of Chinese medicine, Jinzhong 030619, China
2.
Shanxi Key Laboratory of Innovative Drug for the Treatment of Serious Diseases Basing on the Chronic Inflammation, Jinzhong 030619, China

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Received Date: July 19, 2020
Available Online: March 15, 2021

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Abstract

Abstract

Objective: To investigate the purification technology and antioxidation ability of Lycium ruthenicum anthocyanins from Jingle. Methods: Based on the adsorption properties of selected five macroporous resins (HPD100、D101、NKA、AB-8、HPD400) for Lycium ruthenicum anthocyanins from Jingle, the optimum purification resins were screened and the purification parameters were optimized. The antioxidant activity of crude and purified Lycium ruthenicum anthocyanins from Jingle were investigated by DPPH, hydroxyl and ABTS free radical scavenging assay. Results: HPD100 resin had good separation and purification effect. The optimum purification parameters were as follows: Sample concentration 0.2 mg/mL, sample volume 49 mL, elution volume 42 mL with 75% ethanol. After purification, the anthocyanins content was from 2.38% to17.82%. The crude and purified Lycium ruthenicum anthocyanins from Jingle showed good antioxidant activity, the IC₅₀ values of DPPH· scavenging capacity were 0.208 and 0.011 mg/mL respectively, the IC₅₀ values of ABTS⁺· scavenging capacity were 0.476 and 0.064 mg/mL respectively, the IC₅₀ value of purified Lycium ruthenicum anthocyanins for ·OH scavenging capacity was 6.24 mg/mL. Conclusion: The macroporous resin method for purifying Lycium ruthenicum anthocyanins has good purification effect. The antioxidant capacity of the purified Lycium ruthenicum anthocyanins was significantly improved compared with the crude extracts.
- Lycium ruthenicum,
- anthocyanins,
- macroporous resins,
- purification,
- antioxidant

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[1]	乔廷廷, 郭玲. 花青素来源、结构特性和生理功能的研究进展[J]. 中成药,2019,41(2):388−392. doi: 10.3969/j.issn.1001-1528.2019.02.029
[2]	王爱超, 李长兴, 王琦, 等. 黑枸杞花青素对C57BL/6小鼠血糖、血脂及脂肪细胞形态的影响[J]. 青海大学学报,2020,38(3):57−61.
[3]	Zong S, Yang L, Park H J, et al. Dietary intake of Lycium ruthenicum Murray ethanol extract inhibits colonic inflammation in dextran sulfate sodium-induced murine experimental colitis[J]. Food & Function,2020,11:2924−2937.
[4]	Peng Y, Yan Y, Wan P, et al. Effects of long-term intake of anthocyanins from Lycium ruthenicum Murray on the organism health and gut microbiota in vivo[J]. Food Rresearch International,2020,130:108952. doi: 10.1016/j.foodres.2019.108952
[5]	Peng Y, Yan Y, Wan P, et al. Gut microbiota modulation and anti-inflammatory properties of anthocyanins from the fruits of Lycium ruthenicum Murray in dextran sodium sulfate-induced colitis in mice[J]. Free Radical Biology and Medicine,2019,136:96−108. doi: 10.1016/j.freeradbiomed.2019.04.005
[6]	Deng K, Li Y, Xiao M, et al. Lycium ruthenicum Murr polysaccharide protects cortical neurons against oxygen-glucose deprivation/reperfusion in neonatal hypoxic-ischemic encephalopathy[J]. International Journal of Biological Macromolecules,2020,158:562−8. doi: 10.1016/j.ijbiomac.2020.04.122
[7]	Lu K, Wang J, Yu Y, et al. Lycium ruthenicum Murr. alleviates nonalcoholic fatty liver in mice[J]. Food Science & Nutrition, 2020, 8: 2588−2597.
[8]	Peng Q, Liu H, Shi S, et al. Lycium ruthenicum polysaccharide attenuates inflammation through inhibiting TLR4/NF-kappaB signaling pathway[J]. International Journal of Biological Macromolecules,2014,67:330−335. doi: 10.1016/j.ijbiomac.2014.03.023
[9]	同禄禄, 李梦耀, 许小英, 等. HPD-100大孔吸附树脂与聚酰胺吸附黄酮的对比研究[J]. 应用化工,2016,45(5):929−932, 936.
[10]	马乐, 韩军歧, 张润光, 等. 大孔吸附树脂在植物多酚分离纯化中的应用现状[J]. 食品工业科技,2015,36(12):364−367, 374.
[11]	吴艳立, 丁之恩, 闫晗, 等. 黑豆皮中花青素大孔吸附树脂分离纯化工艺研究[J]. 食品研究与开发,2016,37(21):50−53, 67. doi: 10.3969/j.issn.1005-6521.2016.21.012
[12]	赵文娟, 宋扬, 杨洪江. 大孔吸附树脂纯化黑果枸杞中的原花青素[J]. 食品工业科技,2017,38(22):189−194.
[13]	吕玉姣, 尹雨芳, 林强. 黑果枸杞花青素提取分离纯化和组分分析[J]. 化学世界,2016,57(10):659−667.
[14]	顾子杨, 崔梦迪, 李玉华, 等. 黑枸杞花色苷纯化工艺的优化与评价[J]. 中国药理学通报,2018,34(10):1373−1379. doi: 10.3969/j.issn.1001-1978.2018.10.010
[15]	曹叶霞, 王泽慧, 贺金凤, 等. 静乐黑枸杞多糖的提取及抗氧化性分析[J]. 食品工业科技,2019,40(14):196−202.
[16]	郭治羌. 野生黑枸杞人工驯化种植方法[P]. 2015-10-30, 中国, CN201510720316.2.
[17]	杨萍, 李哲. pH示差法与HPLC测定黑枸杞花青素方法比较[J]. 中国农机化学报,2017,38(7):74−78.
[18]	张玲艳, 王宏权. 黑枸杞花青素的提取及其抗氧化活性研究[J]. 食品工业,2014,35(12):88−91.
[19]	熊莹, 樊俊甫, 薛俊文, 等. 茶多酚超声辅助提取工艺优化及抗氧化活性研究[J]. 中国现代应用药学,2020,37(2):175−179.
[20]	郑璐, 胡金贵, 张佳智, 等. 扶芳藤提取物体内体外抗氧化作用研究[J]. 天然产物研究与开发,2020,32(5):742−748.
[21]	Roberta R, Nicoletta P G. Antioxidant activity applying an improved ABTS radical cation decolorization assay[J]. Free Radical Biology and Medicine,1999,26(9/10):1231−1237.
[22]	葡萄皮渣中花旗松素提取工艺优化及其抗氧化能力测定[J]. 食品工业科技, 2021, 42(5): 200-205,220.
[23]	周巾英, 欧阳玲花, 王丽, 等. 白藜芦醇清除DPPH自由基反应的动力学[J]. 食品工业,2020,41(6):222−226.
[24]	王超雪, 陈瑞战, 陆娟, 等. 黑枸杞花青素不同提取工艺及抗氧化活性[J]. 食品工业,2020,41(6):24−28.
[25]	蔡延渠, 董碧莲, 陈利秋, 等. 桃胶多糖体内外抗氧化作用的研究[J]. 食品工业科技,2020,41(13):53−58.
[26]	许瑞如, 张秀玲, 李晨, 等. 微波提取桔梗根多酚工艺优化及抗氧化特性研究[J]. 食品与发酵工业,2020,46(4):187−196.

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