Purification Technology of Pueraria lobata Polysaccharide and Its Antioxidant Activity

Objective:Pueraria lobata was used as raw material to isolate and purify Pueraria lobata polysaccharide by macroporous resin method to study its antioxidant properties. Methods:The optimal resin was selected by adsorption and desorption of polysaccharides from Pueraria lobata,and the effects of adsorption and desorption time,sample solution concentration,sample solution pH and ethanol volume fraction on the purification of polysaccharide were analyzed. Under static analysis conditions,chromatography column method was used for dynamic purification,and the purified Pueraria lobata polysaccharide was analyzed for antioxidant. Results:D101 resin had good separation and purification effect. The best process conditions for the static adsorption and desorption of Pueraria lobata polysaccharides were:Adsorption time 8 h,desorption time 3 h,adsorption concentration 0.75 mg/mL,sample solution pH6.0,ethanol desorption volume fraction was 60%,and the optimal process conditions for dynamic adsorption and desorption was the sample solution mass concentration of 1.0 mg/mL and the desorbent volume(60% ethanol)of 51 mL. Under these condition,the purity of the Pueraria lobata polysaccharide purified reaches 55.34%. The purified Pueraria lobata polysaccharide had a strong scavenging effect on DPPH· and ·OH,the maximum scavenging rates were 81.74% and 85.11%. Conclusion:The macroporous resin method for purifying Pueraria lobata polysaccharides had reasonable technological conditions,good purification effect,high impurity removal rate,and the antioxidant properties of the purified Pueraria lobata polysaccharides.