LI Lin, HUANG Ping-ping, LI Hua-min, WANG Ya-ping, NI Wei-wei. Effect of Different Extraction Methods on the Content of EPA and DHA in Fish Oil by Gas Chromatography[J]. Science and Technology of Food Industry, 2018, 39(15): 255-259. DOI: 10.13386/j.issn1002-0306.2018.15.045
Citation: LI Lin, HUANG Ping-ping, LI Hua-min, WANG Ya-ping, NI Wei-wei. Effect of Different Extraction Methods on the Content of EPA and DHA in Fish Oil by Gas Chromatography[J]. Science and Technology of Food Industry, 2018, 39(15): 255-259. DOI: 10.13386/j.issn1002-0306.2018.15.045

Effect of Different Extraction Methods on the Content of EPA and DHA in Fish Oil by Gas Chromatography

More Information
  • Received Date: December 03, 2017
  • Available Online: November 23, 2020
  • EPA and DHA in fish oil were quantitatively analyzed by gas chromatography(GC),and the analysis conditions for rapid detection of EPA and DHA were explored. The content of EPA and DHA in fish oil from stomach of spanish mackerel fish by ultrasound enhanced subcritical water,ultrasound assisted organic solvent,enzyme hydrolysis and supercritical CO2 extraction were determined using the rapid GC analysis method. The results showed the peak time of EPA was 10.015 min,and DHA was 16.500 min. The heating program and shunt ratio could significantly affect the separation degree of the target peak(EPA,DHA)and its adjacent peak R1 and R2(p<0.01). Compared with the chromatography analysis method of fatty acids recommended in GB5009.168-2016,the method had advantages of strong pertinence(fast detection of EPA and DHA),high accuracy,good reproducibility and high efficiency. The analysis time was shortened from 82 min to 18 min. Under the best analysis conditions,the content of EPA in fish oil were 54.67,35.23,40.13 and 33.40 mg/g,respectively,DHA were 134.01,77.50,102.30 and 67.31 mg/g,respectively.
  • Related Articles

    [1]LAN Tao, WANG Zhiming, ZHAO Lin, LU Shuhuan, SHU Min, LI Xiangyu. Review on the Toxicology of DHA Algal Oil[J]. Science and Technology of Food Industry, 2021, 42(2): 358-363. DOI: 10.13386/j.issn1002-0306.2020040327
    [2]CAO Shaoqian, SUN Cheng, QIN Weili, CHU Yingyan, LI Chongchong, WANG Zhengdong, QI Xiangyang, YANG Hua. Enrichment of EPA and DHA from Pressed Liquor of Fish Meal Processing and Its Characteristics Analysis[J]. Science and Technology of Food Industry, 2021, 42(2): 154-160. DOI: 10.13386/j.issn1002-0306.2020040017
    [3]LIU Jia-wei, WANG Xu, CAO Yan-ping, XU Duo-xia. Research Progress in Technologies of DHA Stabilization[J]. Science and Technology of Food Industry, 2019, 40(7): 273-277. DOI: 10.13386/j.issn1002-0306.2019.07.047
    [4]ZHANG Ming- liang, JIANG Xian- zhang, WANG Can, HUANG Jian- zhong. Research progress in DHA production by microbes[J]. Science and Technology of Food Industry, 2014, (21): 395-400. DOI: 10.13386/j.issn1002-0306.2014.21.077
    [5]ZHANG Ye-feng, HU Yao-chi, HUANG He. Cholesterol removal from microalgae DHA oil[J]. Science and Technology of Food Industry, 2014, (18): 249-252. DOI: 10.13386/j.issn1002-0306.2014.18.046
    [6]ZHAN Qi-ping, ZHU Yu-zhe, ZHANG Xin, WANG Jing-feng. Effects of high levels of DHA and EPA triglyceride on blood lipid and protection of the liver in hyperlipidemia mice[J]. Science and Technology of Food Industry, 2014, (13): 355-358. DOI: 10.13386/j.issn1002-0306.2014.13.068
    [7]YANG Xian-qing, LV Jun-wei, LIN Wan-ling, LI Lai-hao, LI Xiao-yan. Research progress in biological function and antioxidative activity of DHA (Docosahexaenoic acid)[J]. Science and Technology of Food Industry, 2014, (02): 390-394. DOI: 10.13386/j.issn1002-0306.2014.02.069
    [8]MAO Lei, ZHU Yu-zhe, ZHANG Wei-gang, WANG Jing-feng. Effects of high levels of DHA/EPA fish oil triglyceride type on the lipid metabolism in fatty liver rats[J]. Science and Technology of Food Industry, 2013, (24): 347-350. DOI: 10.13386/j.issn1002-0306.2013.24.020
    [9]Perspectives in DHA production of Thraustochytrids[J]. Science and Technology of Food Industry, 2013, (16): 367-371. DOI: 10.13386/j.issn1002-0306.2013.16.002
    [10]Research of application of microalgal DHA in toast[J]. Science and Technology of Food Industry, 2013, (14): 292-295. DOI: 10.13386/j.issn1002-0306.2013.14.014
  • Cited by

    Periodical cited type(1)

    1. 黎永豪. 藻油产量提高措施的研究进展. 食品工业. 2024(09): 165-171 .

    Other cited types(1)

Catalog

    Article Metrics

    Article views (278) PDF downloads (13) Cited by(2)

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return