Optimization of Microwave-assisted Extraction Process of Polysaccharide from <i>Passiflora edulis</i> Sims Peel and Evaluation of Antioxidant Activity <i>in vitro</i>

LI Xia; XIONG Feng; QIN Xian-xing; LI Jia-shu; CUI Meng-jia; HAO Zai-bin; CHEN Hui-ying

doi:10.13386/j.issn1002-0306.2018.15.026

Volume 39 Issue 15

Jul. 2018

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Science and Technology of Food Industry > 2018 > 39(15): 141-146. > DOI: 10.13386/j.issn1002-0306.2018.15.026

LI Xia, XIONG Feng, QIN Xian-xing, LI Jia-shu, CUI Meng-jia, HAO Zai-bin, CHEN Hui-ying. Optimization of Microwave-assisted Extraction Process of Polysaccharide from Passiflora edulis Sims Peel and Evaluation of Antioxidant Activity in vitro[J]. Science and Technology of Food Industry, 2018, 39(15): 141-146. DOI: 10.13386/j.issn1002-0306.2018.15.026

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Optimization of Microwave-assisted Extraction Process of Polysaccharide from Passiflora edulis Sims Peel and Evaluation of Antioxidant Activity in vitro

1. Guangxi Key Laboratory of Electrochemical and Magneto-chemical Functional Materials, Guilin 541004, China;
2. College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, China;
3. Guilin Wanhe Agricultural Products Co., Ltd., Guilin 541000, China

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Received Date: November 12, 2017
Available Online: November 23, 2020

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Abstract

Response surface methodology was applied to optimize the microwave-assisted extraction process of polysaccharide from Passiflora edulis Sims peel and its antioxidant activities in vitro were evaluated. On the basis of single factor test,the ratio of raw material weight to water volume,the extraction time,the microwave power were analyzed and optimized by the Box-Behnken design. The optimized parameters of the microwave-assisted extraction technology were obtained as follows:the ratio of raw material weight to water volume was 1∶27;the extraction time was 3.4 min;the microwave power was 420 W and the extraction rate of polysaccharide was 14.12%±0.41%,which was 1.5 times of that of traditional water bath extraction. Antioxidant activities in vitro of polysaccharide indicated that the DPPH· and ·OH scavenging rates of polysaccharide were 74.02% and 14.41% respectively when the concentration was at 1.0 mg/mL,and the IC₅₀values of 0.374 and 61.06 mg/mL,respectively.
- Passiflora edulis Sims,
- polysaccharide,
- microwave-assisted extraction,
- response surface methodology,
- antioxidant activity

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