贡菊多糖对D-半乳糖致衰老小鼠的抗衰老作用

金声琅; 阙斐; 王莹; 韦笑笑

doi:10.13386/j.issn1002-0306.2015.10.065

贡菊多糖对D-半乳糖致衰老小鼠的抗衰老作用

1. 黄山学院旅游学院
2. 浙江经贸职业技术学院应用工程系
3. 青岛农业大学食品科学与工程学院

基金项目:

2014年度安徽省教育厅自然科学研究重点项目(KJ2014A243)； 2014年度安徽省哲学社会科学规划项目(AHSKQ2014D59)；

详细信息

作者简介:
金声琅 (1980-) , 男, 博士, 副教授, 主要从事农产品精深加工方面的研究。;

中图分类号: R285.5
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- 文章访问数: 176
- HTML全文浏览量: 10
- PDF下载量: 287
出版历程
- 收稿日期: 2014-08-13

Anti-aging effect of total polysaccharide from Dendranthema morifolium on aging mouse induced by D-galactose

Tourism College, Huangshan University
Department of Applied Engineering, Zhejiang Economic and Trade Polytechnic
School of Food Science and Engineering, Qingdao Agricultural University

摘要

摘要: 目的:探讨贡菊多糖对D-半乳糖所致衰老小鼠的抗衰老的作用。方法:将小鼠随机分为5组:空白对照组,D-半乳糖衰老模型对照组（80mg/kg·d bw）,贡菊多糖低、中、高剂量组（50、100、150mg/kg·d bw）,考察血清和肝中SOD、GSH-Px、CAT、T-AOC和血清、肝及脑中的MDA等指标。结果:贡菊多糖能拮抗D-半乳糖所致的小鼠衰老,使小鼠血清及肝组织SOD、GSH-Px、CAT、T-AOC活性明显回升,血清、肝脏和脑组织中MDA的水平下降。结论:贡菊多糖具有延缓衰老的作用。
- 贡菊 /
- 多糖 /
- D-半乳糖 /
- 衰老 /
- 抗氧化
Abstract: Objective:To study the anti-ageing effects of polysaccharide from Dendranthema morifolium on aging mice induced by D-galactose and explore the underlying mechanisms. Methods:Healthy female mice were randomly divided into 5 groups:control group, aging model group (80mg/kg·d bw) , and low-, medium- and high-dose polysaccharide groups (50, 100, 150mg/kg·d bw) . The mice were then sacrificed, and the serum, liver and brain tissue were collected. The concentration of malondialdehyde (MDA) in the blood, liver and brain tissue and the activities of superoxide dismutase (SOD) , glutathione peroxidase (GSH-Px) , Catalase (CAT) , total antioxidant activity (T-AOC) in the serum and liver were then measured. The polysaccharide from Dendranthema morifolium had antagonism effects on the mice aging induced by D-galactose, had evident rebound of activities of SOD, GSH-Px, CAT and T-AOC at all three doses in the blood and liver, and had a decrease of MDA in the serum, liver and brain tissue. Conclusion:The polysaccharide from Dendranthema morifolium had anti-aging effects on aging model mice.
- Dendranthema morifolium /
- polysaccharide /
- D-galactose /
- aging /
- antioxidant

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参考文献(16)

[1]	胡晓倩, 朱洋洋, 陈乐, 等.新鲜黄山贡菊叶中挥发油的提取[J].食品与发酵工业, 2011, 37 (3) :210-214.
[2]	林志超, 余金富, 潘裕添.香蕉皮多糖提取分离纯化及分子质量测定[J].食品科学, 2013, 34 (8) :104-106.
[3]	刘芳, 陈贵堂, 胡秋辉.金针菇锌多糖分离纯化及其结构特征[J].食品科学, 2014, 35 (5) :1-7.
[4]	李正鹏, 吴萍, 孙玉军, 等.树舌胞外富锌多糖体外抗氧化活性研究[J].热带作物学报, 2012, 33 (5) :890-893.
[5]	郑义, 王卫东, 李勇, 等.高良姜多糖提取工艺优化及其抗氧化活性[J].食品科学, 2014, 35 (5) :126-131.
[6]	李姣, 王珂, 王瑞坡, 等.芦笋多糖提取纯化工艺及其体外抗氧化研究[J].食品科学, 2011, 32 (8) :65-69.
[7]	徐洁昕, 方红霞, 楚文靖, 等.黄山贡菊多糖的微波浸提工艺和抑菌效果研究[J].食品工业科技, 2011, 32 (9) :280-283.
[8]	于慧, 王锡昌, 奚印慈.甜叶菊废弃物制得生物制剂的抗氧化活性研究[J].食品科学, 2008, 29 (8) :65-69.
[9]	中国食品药品监督管理局.保健食品抗氧化功能评价方法[S].2012.
[10]	倪爱威, 崔桂友, 陆广念, 等.马齿苋提取物对D-半乳糖致衰老的小鼠体内抗氧化效应[J].营养学报, 2010, 32 (3) :297-298.
[11]	余强, 聂少平, 李文娟, 等.黑灵芝多糖对D-半乳糖致衰老小鼠的作用研究[J].食品科学, 2009, 30 (17) :305-307.
[12]	闫倩, 俞龙泉, 陈野, 等.草果甲醇溶出物对D-半乳糖致衰老小鼠的抗氧化作用机理研究[J].食品工业科技, 2014, 35 (6) :351-356.
[13]	龚国清, 徐黻本.小鼠衰老模型研究[J].中国药科大学学报, 1991, 22 (2) :101-103.
[14]	任旭, 陈贵林.唐古特白刺果实提取物抗氧化活性评价[J].食品科学, 2011, 32 (3) :95-97.
[15]	Kirakosyan A, Seymour e M, Noon K R, et al.Interactions of antioxidants isolated from tart cherry (Prunus cerasus) fruits[J].Food Chemistry, 2010, 122 (1) :78-83.
[16]	Ardestani A, Yazdanparast R.Antioxidant and free radical scavenging potential of Achillea santolina extracts[J].Food Chemistry, 2007, 104:21-29.