Effect of Different Harvesting Times on Total Flavonoids Content and Antioxidant Activity in Rosae Chinensis Flos
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摘要: 目的:通过比较月季花不同采收期总黄酮含量与其抗氧化活性,确定月季花的最佳采收期,为月季花临床用药的有效性奠定理论基础。方法:采用回流提取法提取月季花提取物,紫外分光光度法测定月季花总黄酮含量,以清除1,1-二苯基-2-苦基肼自由基(DPPH·)及羟基自由基(OH·)的能力来评价不同采收期月季花抗氧化活性。结果:不同采收期总黄酮含量变化为:4月份>5月份>9月份>10月份>11月份>6月份>7月份>8月份。以4月份采收月季花总黄酮含量最高,含量达到(152.28±1.69) mg/g。不同采收期月季花提取物均有较好的抗氧化活性,其中7月份采收的月季花提取物体外抗氧化活性最强。结论:月季花提取物中总黄酮含量与其抗氧化活性正相关,但其抗氧化活性受采收期和提取物中的其他杂质的影响。Abstract: Objective:The best harvest time was determined by comparing the content of total flavonoids and antioxidant activity of Rosae Chinensis Flos in different harvest time,which laid a theoretical foundation for the effectiveness of clinical medication of Rosae Chinensis Flos. Methods:The total flavonoids of Rosae Chinensis Flos were extracted by reflux extraction and determined by UV spectrophotometry. The antioxidant activity of Rosae Chinensis Flos was evaluated by scavenging DPPH· and OH·. Results:The change in content of total flavonoids in different harvest time was:April>May>September>October>November>June>July>August. The content of total flavonoids from rose flowers was the highest in April,which was(152.28±1.69) mg/g. The total flavonoids from Rosae Chinensis Flos in different harvesting times exhibited good antioxidant activity. Among them,the best antioxidant activity was observed in total flavonoids from Rosae Chinensis Flos harvested in July. Conclusion:There was positive correlation between the content of total flavonoids and antioxidant activity in Rosae Chinensis Flos. However,its antioxidant activities were affected by harvesting time and other impurities.
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