响应面法优化超声辅助提取代代花总黄酮的工艺及其抗氧化活性研究

郝可欣; 胡文忠; 张清洁; 王澳盛; 于皎雪; 郭斌梅; 侯梦阳

doi:10.13386/j.issn1002-0306.2019.24.026

响应面法优化超声辅助提取代代花总黄酮的工艺及其抗氧化活性研究

郝可欣^1,2,
胡文忠^1,2, ,,
张清洁^1,2,
王澳盛^1,2,
于皎雪^1,2,
郭斌梅^1,2,
侯梦阳^2,3

1. 大连民族大学生命科学学院, 辽宁大连 116600;
2. 生物技术与资源利用教育部重点实验室, 辽宁大连 116600;
3. 大连理工大学生命科学与技术学院, 辽宁大连 116024

基金项目:

"十三五"国家科技支撑计划项目(2016YFD0400903)

国家自然科学基金(31471923)

大连民族大学-铜仁科学院联合科研项目(TR02004)。

详细信息

作者简介:
郝可欣(1996-),女,硕士研究生,研究方向:天然产物提取与利用,E-mail:haokex@sina.com。

通讯作者:
胡文忠(1959-),男,博士,教授,研究方向:民族药开发应用及农产品贮藏加工,E-mail:wenzhongh@sina.com。

中图分类号: TS283.6
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出版历程
- 收稿日期: 2019-04-01
- 网络出版日期: 2020-11-12
- 刊出日期: 2019-12-14

Optimization of the Ultrasonic-assisted Extraction of Total Flavonoids from Citrus aurantium L. var daidai by Response Surface Methodology and Its Antioxidant Activity

1. College of Life Science, Dalian Nationalities University, Dalian 116600, China;
2. Key Laboratory of Biotechnology and Bioresources Utilization, Ministry of Education, Dalian 116600, China;
3. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

摘要

摘要: 采用响应面法优化超声辅助提取代代花总黄酮的工艺,并研究代代花总黄酮的抗氧化活性。本实验首先通过单因素实验,考察乙醇浓度、料液比、提取温度及时间对总黄酮得率的影响,然后采用四因素三水平响应面试验设计优化代代花总黄酮的最佳提取工艺,同时通过DPPH·和·OH的清除实验对代代花总黄酮的抗氧化活性进行评估。结果表明,最佳提取工艺条件是乙醇浓度60%、液料比20:1 mL/g、提取时间35 min、提取温度为60℃,该条件下代代花总黄酮的得率为2.62%,该值与预测值2.66%高度相符;其对DPPH·和·OH均有较强的清除作用,IC₅₀值分别为0.385和0.255 mg/mL。通过响应面法优化的代代花总黄酮超声辅助提取工艺稳定可行,且代代花总黄酮提取物具有较强的抗氧化活性。
- 代代花 /
- 总黄酮 /
- 超声辅助提取 /
- 响应面法 /
- 抗氧化活性
Abstract: To optimize the ultrasonic-assisted extraction technology of total flavonoids from Citrus aurantium L. var daidai by response surface method and study its antioxidant activity. Firstly,the effects of ethanol concentration,solid-liquid ratio,extraction temperature and time on the extraction yield of total flavonoids by single factor experiment,then the extraction process was optimized by four-factor and three-level design of surface response analysis,at the same time,the antioxidant activity of flavonoids from Citrus aurantium L. var daidai was studied by assessing the DPPH· and ·OH scavenging activities. Results showed that the optimum process conditions for the extraction total flavonoids were as follows:ethanol concentration 60%,the ratio of liquid to solid 20:1 mL/g,extraction time 35 min,extraction temperature was 60 ℃,the extraction yield of total flavonoids from Citrus aurantium L. var daidai was 2.62% under such conditions,which was highly consistent with the predicted value of 2.66%;and the IC₅₀ values of DPPH· and ·OH scavenging activities were 0.385 and 0.255 mg/mL,respectively. The extraction process optimized by response surface method was stable and feasible,and the total flavonoids from Citrus aurantium L. var daidai had strong antioxidant activity.
- Citrus aurantium L. var daidai /
- total flavonoids /
- ultrasonic-assisted extraction /
- response surface methodology /
- antioxidant

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