玉米浆粉预处理对粘质沙雷氏菌发酵产2-酮基-D-葡萄糖酸的影响

Effects of pretreatment with corn steep powder on the fermentation of 2-keto-D-gluconic acid by Serratia marcescens

摘要: 通过比较不同预处理方法获得的玉米浆粉在粘质沙雷氏菌SD136作用下产2-酮基-D-葡萄糖酸的影响,组合复配玉米浆粉,结果表明:最佳玉米浆粉复配比为玉米原浆粉和酶解玉米浆粉3∶1,2-酮基-D-葡萄糖酸产量达到(171.39±4.5) g/L,发酵时间由40 h缩短至37 h,对糖转化率达到95.22%。玉米原浆粉和酶解玉米浆粉的组合复配能够提高粘质沙雷氏菌SD136的发酵效率和2-酮基-D-葡萄糖酸的产量。

Abstract: By comparing the effects of corn steep powder with different pretreatment methods on the production of 2-keto-D-gluconic acid by Serratia marcescens SD136,corn steep powder were compounded.The results showed that the optimum ratio of corn steep powder and enzymolysis corn steep was 3∶1,the yield of 2-keto-D-gluconic acid increased to(171.39±4.5) g/L,the fermentation period shortened from 40 h to 37 h,and the sugar conversion rate reached 95.22%. The combination of corn steep powder and enzymolysis corn steep powder can improve the fermentation efficiency of Serratia marcescens SD136 and the production of 2-keto-D-gluconic acid.