Abstract: The composite beverage was developed with Punica granatum fruit and polysaccharide of Amaranthus caudatus L. as main raw materials and observed its anti-fatigue activity. Sensory evaluation score was used as the index. The single factor experiments and response surface analysis method were used to optimize the formula of composite beverage, and investigateits effect of exhaustive swimming time and related biochemical indexes after animal exercise. The results showed that the best formula of the compound beverage was as follows: The volume ratio of 10% Prunus syringae polysaccharide liquid to 20% Punica granatum juice was 2:5, the amount of sucrose was 4.5%, the amount of citric acid was 0.4%, the amount of sodium carboxymethyl cellulose was 0.06%, and the sensory score was 89.8. Compared with the blank control group, the animal groups of composite beverage at middle and high dose could significantly prolong the exhaustive swimming time (P<0.01), obviously decreased BLA (P<0.01) and BUN (P<0.01) contents after exercise. Therefore, the composite beverage of Amaranthus caudatus L. and Punica granatum had better anti-fatigue function.