Abstract: Objective: To investigate the effects of Cerasus humilis composite rod on fatigue resistance and energy metabolism in mice. Methods: Fifty healthy male mice at 8 weeks of age were randomly divided into 5 groups: Control group, model group, Cerasus humilis composite rod group, positive group and high-fat group. By establishing a fatigue model of swimming+diet control, after 21 days of continuous feeding, the fatigue, energy metabolism and antioxidant related indexes of mice were detected by climbing poles and exhausting swimming time. Results: Compared with the control group, the climbing time and swimming time of the model group decreased significantly (P<0.01), the contents of lactate, blood urea nitrogen, creatine kinase and malondialdehyde were significantly increased (P<0.01), and serum superoxide dismutase, hepatic Na⁺-K⁺-ATPase and Ga²⁺-Mg²⁺-ATPase decreased significantly (P<0.05). Compared with the model group, the Cerasus humilis composite rod group and positive group could significantly prolong the time of climbing the pole and the time of swimming to exhaustion, which were 2.1 and 1.8 times that of the model group, and could effectively reduce energy consumption, reduce the accumulation of lactate and blood urea nitrogen (P<0.01, P<0.05), reduce the content of MDA and CK in serum, enhance the activity of SOD enzyme in serum (P<0.01), increase the activities of Na⁺-K⁺-ATPase and Ga²⁺-Mg²⁺-ATPase in liver (P<0.01), increase the level of ATP content and exert anti-fatigue activity. Conclusion: The composite rod has significant anti-fatigue ability and energy metabolism ability, which can improve the exercise ability level of mice by increasing the pole climbing time and weight-bearing exhaustion swimming time, and alleviate fatigue by reducing energy consumption, increasing energy metabolism, improving exercise endurance and improving antioxidant capacity.