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中国精品科技期刊2020
闫丹,孔云菲,孙英明,等. 动物肝脏均质液的添加对鱼糜凝胶特性的影响[J]. 食品工业科技,2023,44(12):55−61. doi: 10.13386/j.issn1002-0306.2022080278.
引用本文: 闫丹,孔云菲,孙英明,等. 动物肝脏均质液的添加对鱼糜凝胶特性的影响[J]. 食品工业科技,2023,44(12):55−61. doi: 10.13386/j.issn1002-0306.2022080278.
YAN Dan, KONG Yunfei, SUN Yingming, et al. Effects of Liver Homogenate Addition on the Gel Properties of Surimi[J]. Science and Technology of Food Industry, 2023, 44(12): 55−61. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080278.
Citation: YAN Dan, KONG Yunfei, SUN Yingming, et al. Effects of Liver Homogenate Addition on the Gel Properties of Surimi[J]. Science and Technology of Food Industry, 2023, 44(12): 55−61. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080278.

动物肝脏均质液的添加对鱼糜凝胶特性的影响

Effects of Liver Homogenate Addition on the Gel Properties of Surimi

  • 摘要: 为了研究畜禽动物肝脏的添加对鱼糜凝胶特性的影响,本研究选取猪肝和鸭肝制备均质液,分别以5%或10%的比例添加入鲢鱼鱼糜中,并测定分析混合鱼糜凝胶的质构特性、流变学性质、水分分布、分子间作用力等。结果显示,肝脏均质液的加入提高了鱼糜凝胶的硬度以及储能模量(G')和损耗模量(G''),降低了凝胶的白度值、回复性和内聚性,对凝胶体系中水分分布未见显著影响;随着肝脏均质液添加量由5%提高到10%,鱼糜凝胶的硬度未呈现显著变化,而白度、回复性和内聚性则进一步下降;鸭肝均质液的TG酶粗酶活显著高于猪肝组,5%添加量下,破坏体系中离子键、氢键、疏水键、二硫键之后鸭肝混合鱼糜凝胶中蛋白质溶解性高于猪肝组,SDS-PAGE分析显示添加鸭肝组的样品相比猪肝组其肌球蛋白重链条带变浅,可能是更多的肌球蛋白参与了TG酶介导的分子间共价交联所致;10%添加量的情况下,猪肝组鱼糜凝胶强度以及不易流动水的比例相比对照组显著下降,而鸭肝组未见差异。初步研究表明5%猪肝均质液的添加对冷冻鲢鱼鱼糜凝胶的性能有显著影响,可能与其中含有TG酶有关,需要进一步研究揭示。

     

    Abstract: In order to study the effects of animal liver addition on the gel properties of surimi, homogenized pig liver or duck liver was added to silver carp surimi at a level of 5% or 10%, respectively. The texture profile analysis, rheological properties, water distribution and intermolecular force of mixed surimi gel were determined. Results showed that with the addition of liver homogenates, the hardness, storage modulus (G') and loss modulus (G'') of surimi gel increased. And the whiteness, resilience and cohesiveness decreased, while water distribution was not greatly affected. When the content of liver homogenate increased from 5% to 10%, hardness did not change while whiteness, resilience and cohesiveness decreased further. The crude enzyme activity of transglutaminase (TGase) in duck liver homogenate was significantly higher than that of pig liver. At 5% addition level, protein resolubility of duck liver mixed surimi gel was higher than that of pig liver gel after breaking ionic, hydrogen, hydrophobic and disulfide bonds in the system. SDS-PAGE analysis showed that the myosin heavy chain in the duck liver group was less dense than that in the pig liver group, which might due to a greater involvement of myosin in TGase-mediated intermolecular cross-linking. Compared with the control group, the gel strength and the proportion of immobilized water in the pig liver group decreased significantly, while there was no difference in the duck liver group. The preliminary results showed that the addition of 5% pig liver homogenate had a significant effect on the gel properties of frozen silver carp surimi, which might be related to the endogenous TGase in the liver. However, this requires further investigation.

     

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