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中国精品科技期刊2020
尹丽,韦小敏,卢成淑,等. 半枫荷根对佐剂诱导型关节炎的改善作用及其正丁醇提取物UPLC-Q-TOF-MS分析[J]. 食品工业科技,2022,43(22):396−405. doi: 10.13386/j.issn1002-0306.2022040259.
引用本文: 尹丽,韦小敏,卢成淑,等. 半枫荷根对佐剂诱导型关节炎的改善作用及其正丁醇提取物UPLC-Q-TOF-MS分析[J]. 食品工业科技,2022,43(22):396−405. doi: 10.13386/j.issn1002-0306.2022040259.
YIN Li, WEI Xiaomin, LU Chengshu, et al. Effects of Semiliquidambar cathayensis Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS[J]. Science and Technology of Food Industry, 2022, 43(22): 396−405. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040259.
Citation: YIN Li, WEI Xiaomin, LU Chengshu, et al. Effects of Semiliquidambar cathayensis Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS[J]. Science and Technology of Food Industry, 2022, 43(22): 396−405. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022040259.

半枫荷根对佐剂诱导型关节炎的改善作用及其正丁醇提取物UPLC-Q-TOF-MS分析

Effects of Semiliquidambar cathayensis Root on Adjuvant Induced Arthritis in Rats and Analysis of Its n-Butanol Extract by UPLC-Q-TOF-MS

  • 摘要: 目的:本研究探讨了半枫荷根对类风湿性关节炎的治疗作用,明确活性提取物及化学成分。方法:建立SD大鼠佐剂性关节炎模型,测定侧足肿胀容积、关节炎指数变化、胸腺、脾脏指数;ELISA法测定外周血单核细胞(PBMC)中COX-2、5-LOX及血清IL-6、IL-1β、TNF-α、IL-10水平;HE染色观察踝关节病理组织形态,用上述指标筛选半枫荷根活性提取物,UPLC-Q-TOF-MS技术对活性提取物进行定性。结果:与模型组比,半枫荷根醇提物组(1.6 g/kg)的大鼠侧足肿胀度、关节炎指数、脾脏指数降低;COX-2及5-LOX、血清IL-6、IL-1β、TNF-α水平明显降低,IL-10水平明显升高;关节组织炎细胞浸润减少、病理形态改善;半枫荷根正丁醇部位为活性提取物;正丁醇提取物鉴定得到104个成分,包括有机酸类、糖和糖苷类、黄酮类、醌类及香豆素等有机化合物。结论:半枫荷根对佐剂型关节炎具有明显保护作用,在治疗药物和功能性食品的开发方面有广阔的前景。

     

    Abstract: Objective: The effect of the Semiliquidambar cathayensis root on adjuvant-induced arthritis in rats was investigated, and the active extract and the chemical components in the root were determined. Methods: Rheumatoid arthritis model rats were divided randomly into five groups. Changes in the degree of swelling of the left hind feet and arthritis indexes were recorded. The thymus and spleen indexes of the rats were calculated after treatment. Changes in the COX-2 and 5-LOX levels in peripheral blood monocytes (PBMC) and serum IL-6, IL-1β, TNF-α, and IL-10 levels were measured. The pathomorphological changes to the rat ankle joint were observed by hematoxylin-eosin staining(HE staining). The effects of four different solvent fractions of an alcohol extract of the Semiliquidambar cathayensis root on changes to the left hind foot swelling volume and arthritis index in rats were investigated. The effective parts were analyzed qualitatively by UPLC-Q-TOF-MS. Results: The degree of foot swelling, arthritis indexes, and spleen indexes were reduced significantly in the positive drug group (1.6 g/kg) when compared with the model group. The COX-2 and 5-LOX levels in PBMC and IL-6, IL-1β, and TNF-α decreased significantly. The IL-10 serum level increased significantly. The infiltration of inflammatory cells in the joint tissue decreased, and morphology improved. The swelling volume of lateral feet and arthritis index in rats were reduced significantly in the n-butanol fraction from total alcohol extracts of the Semiliquidambar cathayensis root. One-hundred and four chemical components were identified from the n-butanol fraction, including organic acids, sugars and glycosides, flavonoids, quinones, and coumarins. Conclusion: The Semiliquidambar cathayensis root had an apparent protective effect on adjuvant arthritis-immunized rats. This root would have broad prospects in developing antiarthritic drugs and functional food.

     

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