Abstract: Objective: To investigate the bactericidal effect of acidic electrolyzed-oxidizing water treatment on the surface of fresh-cut ginseng slices and the effect on the surface color of fresh-cut ginseng slices, so as to obtain the optimal sterilization process conditions and provide a new method for fresh ginseng preservation. Method: Firstly, the bactericidal rate of fresh-cut ginseng slices was taken as the index to investigate the influence of four factors (solid-liquid ratio, soaking time, soaking temperature and pH) on the bactericidal effect. Then, a three-factor and three-level response surface optimization experiment was used to optimize the bactericidal process, and the color and luster of fresh-cut ginseng slices treated with acidic electrolyzed-oxidizing water were measured. Results: The best technological conditions for treating fresh ginseng slices with acidic oxidizing potential water were as follows: Solid-liquid ratio 1:10 g/mL, soaking temperature 25 ℃ and soaking time 11 min. Under these conditions, the bactericidal rate was 94.40%±0.61%. The significance order of the influencing factors was soaking time >solid-liquid ratio > soaking temperature. The surface color of fresh-cut ginseng slices had no obvious change before and after acidic electrolyzed-oxidizing water treatment. Compared with other common fungicides, the fungicide efficiency of acidic electrolyzed-oxidizing water increased by 24.5%~30.7%. Conclusion: Acidic electrolyzed-oxidizing water treatment technology had a significant bactericidal effect on the surface of fresh-cut ginseng slices, which could provide a more effective, safer and high-quality non-thermal sterilization method for the preservation and processing of ginseng.