Abstract: This study aimed to explore the hypoglycaemic effects of the compound Pueraria lobate and Ophiopogon japonicus tea. In this study, the compound Pueraria lobate and Ophiopogon japonicus tea was administered to normal mice. Moreover, a mouse model of hyperglycemia was established, and high (3.0 g/kg BW), medium (2.0 g/kg BW), and low (1.0 g/kg BW) doses of compound Pueraria lobate and Ophiopogon japonicus tea were administered to mice with hyperglycemia. The body weight, blood glucose and glucose tolerance indexes of the mice were detected. In addition, the control group was given placebo and the experimental group was given compound Pueraria lobate and Ophiopogon japonicus tea twice a day, 3 g each time for 60 days in hyperglycemic people. The changes in glycosylated hemoglobin, blood glucose and clinical symptoms were observed. The results showed that compound Pueraria lobate and Ophiopogon japonicus tea had no effect on the body weight and blood glucose in normal mice, but the 2 h blood glucose of hyperglycemic model mice in high and medium dose groups were decreased significantly and extremely significantly, respectively (P<0.05, P<0.01). The area under the blood glucose curve of hyperglycemic model mice in the high dose group was significantly decreased (P<0.05). The results of clinical trials showed compound Pueraria lobate and Ophiopogon japonicus tea could significantly reduce fasting blood glucose and 2 h blood glucose in hyperglycemic people (P<0.01). The effective rate of blood glucose reduction was 70.91%, and the effective rate of improving the symptoms of thirst, polyuria, polydipsia, hunger, fatigue was more than 44.90%. In conclusion, the compound Pueraria lobate and Ophiopogon japonicus tea had hypoglycaemic activity. The study had important significance and value for the prevention and treatment of diabetes and its complications.