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中国精品科技期刊2020
张孟琴,徐路,张俊波,等. 三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价J. 食品工业科技,2022,43(10):388−394. doi: 10.13386/j.issn1002-0306.2021090348.
引用本文: 张孟琴,徐路,张俊波,等. 三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价J. 食品工业科技,2022,43(10):388−394. doi: 10.13386/j.issn1002-0306.2021090348.
ZHANG Mengqin, XU Lu, ZHANG Junbo, et al. Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its ExtractsJ. Science and Technology of Food Industry, 2022, 43(10): 388−394. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090348.
Citation: ZHANG Mengqin, XU Lu, ZHANG Junbo, et al. Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its ExtractsJ. Science and Technology of Food Industry, 2022, 43(10): 388−394. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021090348.

三叶木通果皮主要营养成分、活性成分含量测定及果皮提取物抗氧化活性评价

Determination of Contents of the Main Nutritional Components, Functional Components of Akebia trifoliata Pericarp and the Antioxidant Activity of Its Extracts

  • 摘要: 为了探究三叶木通果皮的营养价值及活性功能,本研究采用凯氏定氮法、酸水解法、苯酚硫酸法、3,5-二硝基水杨酸法、ICP-AES、亚硝酸钠-三氯化铝法、福林酚法和香草醛-高氯酸等方法,测定了三叶木通果皮中的蛋白质、脂肪、总糖、还原糖、8种矿质元素及粗纤维素等主要营养成分与总黄酮、总酚及总皂苷等活性物质的含量,并研究了果皮提取物的抗氧化活性。结果表明,三叶木通果皮中蛋白质(8.16%±0.11%)、还原糖(19.31%±0.21%)、总糖(32.61%±0.18%)等主要营养成分的含量均较高,其中,果胶含量达到(20.08%±0.20%);所测8种营养元素中,K含量最高(14061.17 µg/g DW),其次为Ca(4959.28 µg/g DW),8种元素含量高低顺序为K>Ca>Mg>Fe>Mn>Na>Cu>Zn。果皮中总黄酮、总酚及总皂苷等活性物质的含量分别为(20.58±0.12)、(31.53±0.18)、(45.20±12.04)mg/g DW;三叶木通果皮提取物清除DPPH自由基、ABTS+自由基的IC50分别为0.049、0.052 mg/mL ,且清除能力与果皮提取物的质量浓度呈现出良好的量效关系。果皮中总黄酮、总酚、总皂苷的含量与抗氧化活性均呈现极显著正相关(P<0.01)。综上,三叶木通果皮是一种营养价值较高、具有一定抗氧化活性的功能型新材料。

     

    Abstract: In order to explore the nutritional value and active function of Akebia trifoliata pericarp, the main nutritional components contents of protein, fat, total sugar, reducing sugar, 8 mineral elements, total flavonoids, total phenols and total saponins were determined by using Kjeldahl method, acid hydrolysis, phenol sulfuric acid method, 3,5-dinitrosalicylic acid method, ICP-AES method, NaNO2-AlCl3 method, Folin-ciocalten and vanillin-perchloric acid. The antioxidant capacity of pericarp extracts was also evaluated. The results showed that the contents of protein (8.16%±0.11%), reducing sugar (19.31%±0.21%) and total sugar (32.61%±0.18%) in the peel of Akebia trifoliata were high, and the pectin content determined by gravimetric method was (20.08%±0.20%). Among the eight nutrient elements measured, K content was the richest (14061.17 μg/g DW), followed by Ca (4959.28 μg/g DW). The orders of the contents of eight mineral elements were K>Ca>Mg>Fe>Mn>Na>Cu>Zn. The contents of total flavonoids, polyphenolics and saponins were (20.58±0.12), (31.53±0.18) and (45.20±12.04) mg/g DW, respectively. The scavenging capabilities of extraction on the free radicals of DPPH and ABTS+ were also investigated. When the scavenging rate reached to 50%, the IC50 values on DPPH and ABTS+ free radicals scavenging were 0.049, 0.052 mg/mL, respectively. The scavenging ability of extracts in Akebia trifoliata pericarp was dose-dependent. The contents of total flavonoids, total polyphenols and total saponins had strong and positive correlations with antioxidant activity (P<0.01). In summary, Akebia trifoliata pericarp should be new resources with high nutritional values and strong antioxidant activities worth for in-depth development.

     

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