Abstract: Lactic acid bacteria are of great benefit to human life and are extremely important research objects in industrial and commercial production. High-density culture of lactic acid bacteria is an important step for its industrial application. High-density culture of lactic acid bacteria can obtain higher bacterial density with lower culture volume and shorter culture cycle, improve fermentation speed and fermentation effect, and reduce the subsequent use of starter, control equipment investment and reduce production cost in production practice. High-density culture of lactic acid bacteria is affected by factors such as production strains, medium, fermentation conditions, and fermentation modes. This paper mainly reviewed nutrient consumption mode, medium, culture condition, culture technology from high-density culture of lactic acid bacteria. Finally, the future research directions were prospected, in order to provide theoretical basis for the efficient preparation and industrial application of lactic acid bacteria starter.