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中国精品科技期刊2020
关博洋,殷菲胧,刘云芬,等. 贮藏温度对采后龙眼果实糖代谢及其相关酶活性的影响[J]. 食品工业科技,2022,43(5):348−355. doi: 10.13386/j.issn1002-0306.2021060261.
引用本文: 关博洋,殷菲胧,刘云芬,等. 贮藏温度对采后龙眼果实糖代谢及其相关酶活性的影响[J]. 食品工业科技,2022,43(5):348−355. doi: 10.13386/j.issn1002-0306.2021060261.
GUAN Boyang, YIN Feilong, LIU Yunfen, et al. Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits[J]. Science and Technology of Food Industry, 2022, 43(5): 348−355. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060261.
Citation: GUAN Boyang, YIN Feilong, LIU Yunfen, et al. Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits[J]. Science and Technology of Food Industry, 2022, 43(5): 348−355. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2021060261.

贮藏温度对采后龙眼果实糖代谢及其相关酶活性的影响

Effects of Storage Temperature on Sugar Metabolism and Related Enzyme Activities of Postharvest Longan Fruits

  • 摘要: 研究采后不同贮藏温度(25、15、4 ℃)对‘石硖’龙眼果实糖组分及相关酶活性的影响。测定龙眼果肉可溶性固形物含量(SSC)、3种主要糖含量和糖代谢相关酶活性的变化,对不同贮藏温度各个时期样品进行主成分分析和载荷因子分析,并对各指标进行相关性分析。结果表明:25 ℃贮藏的龙眼SSC下降最快,贮藏8 d即从23.5%降至22.0%,且蔗糖含量下降最快,葡萄糖和果糖含量呈上升趋势;15 、4 ℃均能有效延缓SSC的下降,4 ℃贮藏的龙眼SSC下降更慢且蔗糖和葡萄糖含量下降的最慢,但果糖含量的下降快于15 ℃。不同温度贮藏龙眼果肉的酸性转化酶(AI)、中性转化酶(NI)和蔗糖合成酶(SS)活性均随着贮藏时间的延长升高,25 ℃上升的幅度和速度最快,15 ℃次之,4 ℃上升的最平缓,表明低温显著抑制了蔗糖代谢酶活性的升高。相关性分析表明,不同温度贮藏的龙眼蔗糖含量与SSC呈极显著正相关,表明SSC一定程度上可反映蔗糖含量;蔗糖含量与葡萄糖含量呈显著正相关,同时与AI、NI、SS呈显著负相关,推测葡萄糖和果糖由蔗糖降解而来;葡萄糖含量和果糖含量呈极显著正相关,但二者在15 ℃和4 ℃贮藏果实中下降规律不一致,暗示二者的消耗有所差异。以上结果说明,低温可以明显抑制龙眼果实糖代谢和相关酶活性的升高从而减缓贮藏过程中糖分的分解,延长贮藏时间,其中,4 ℃贮藏效果最佳。

     

    Abstract: To study the effects of different storage temperatures (25, 15, 4 ℃) after harvest on the sugar components and related enzyme activities of "Shixia" longan fruits. The longan pulp soluble solid content (SSC), the content of three main sugars and the changes in the activities of sugar metabolism-related enzymes were measured. Principal component analysis and load factor analysis were performed on samples at different storage temperatures and various periods, and the correlation analysis of each index was performed. The results showed that the SSC of longan stored at 25 ℃ decreased the fastest, from 23.5% to 22.0% after 8 days of storage, and the sucrose content decreased the fastest, but the glucose and fructose content showed an upward trend; both 15℃ and 4 ℃ could effectively delay SSC. The decrease in SSC of longan stored at 4 ℃ was slower and the sucrose and glucose content decreased the slowest, but the decrease in fructose content was faster than 15 ℃. The acid invertase (AI), neutral invertase (NI) and sucrose synthase (SS) activities of longan pulp stored at different temperatures all increase with the storage time, the amplitude and speed of the increase was the fastest at 25 °C, at 15 °C was secondly, and the 4 ℃ rise was the most gentle, indicating that low temperature significantly inhibited the increase in the activity of sucrose metabolizing enzymes. Correlation analysis showed that the sucrose content of longan stored at different temperatures had a very significant positive correlation with SSC, indicating that SSC could reflect the sucrose content to a certain extent; the sucrose content had a significant positive correlation with the glucose content, and it also had significant negative correlation with AI, NI, and SS. It was inferred that glucose and fructose were degraded from sucrose; glucose content and fructose content were extremely significantly positively correlated, but the two decline patterns in 15 ℃ and 4 ℃ storage fruits were inconsistent, suggesting that the consumption of the two was different. The above results indicate that low temperature could significantly inhibit the increase of sugar metabolism and related enzyme activities in longan fruit, thereby slowing down the decomposition of sugar during storage and prolonging the storage time. Among them, the storage effect at 4 ℃ was the best.

     

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